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Author:
Date:
2011-06-20
[Abstract] Intraperitoneal (i.p.) injection of thioglycollate elicits a robust influx of neutrophils into peritoneal cavity. The trafficking of the cells is believed to be mediated by chemokines CXCL1, CXCL2, and CXCL8 (Call et al., 2001; Cacalano et al., 1994). Thus this model can be used to test the ability of neutrophils to migrate towards these chemokines in bioengineered mouse strains (e.g. knockout or transgenic mice) or the ability of certain molecules to inhibit the chemoattractive activities of these chemokines (e.g. small molecules or inhibitory antibodies). This protocol has been used by the author successfully to test the functions of a viral multi-chemokine inhibitor.
[摘要] 腹膜内（i.p.）注射巯基乙酸盐引发嗜中性粒细胞进入腹膜腔的强烈内流。 认为细胞的运输由趋化因子CXCL1，CXCL2和CXCL8介导（Call等人，2001; Cacalano等人，1994）。 因此，该模型可用于测试中性粒细胞在生物工程化小鼠品系（例如敲除或转基因小鼠）中向这些趋化因子迁移的能力或某些分子抑制这些趋化因子的化学吸引活性的能力（例如， 例如小分子或抑制性抗体）。 该方案已被作者成功地用于测试病毒多趋化因子抑制剂的功能。
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