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Author:
Date:
2012-02-20
[Abstract] If an antibody for your protein of interest is available, immunofluorscence is a useful method to detect the localization and relative abundance of the protein by using a fluorescence microscope. Immunofluoresence can be used in combination with other, non-antibody methods of fluorescence staining, for example, the use of DAPI to label DNA. This protocol describes setting up an immunofluorescence experiment using cells grown on a coverglass.
[摘要] 如果您感兴趣的蛋白质的抗体是可用的，免疫荧光是一种有用的方法，通过使用荧光显微镜检测蛋白质的本地化和相对丰度。 免疫荧光可以与其他非抗体荧光染色方法组合使用，例如，使用DAPI标记DNA。 该协议描述了使用生长在盖玻片上的细胞建立免疫荧光实验。
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