| Primary Culture of Mouse Neurons from the Spinal Cord Dorsal Horn
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Author:
Date:
2017-01-05
[Abstract] Primary afferents of sensory neurons mainly terminate in the spinal cord dorsal horn, which has an important role in the integration and modulation of sensory-related signals. Primary culture of mouse spinal dorsal horn neuron (SDHN) is useful for studying signal transmission from peripheral nervous system to the brain, as well as for developing cellular disease models, such as pain and itch. Because of the specific features of SDHN, it is necessary to establish a reliable culture method that is suitable for testing neural response to various external stimuli in vitro.
[摘要] 感觉神经元的主要传入主要终止于脊髓背角，其在感觉相关信号的整合和调节中具有重要作用。小鼠脊髓背角神经元（SDHN）的原代培养可用于研究从周围神经系统到脑的信号传递，以及用于发展诸如疼痛和瘙痒的细胞疾病模型。由于SDHN的具体特征，有必要建立一种可靠的培养方法，适合于测试体外各种外部刺激的神经反应。
背景 不同于目前用于培养分离的小鼠来自海马或大脑皮质的原代神经元的方案，报道了很少的在体外培养SDHN的方法。该协议主要基于以前描述的方法（Hu et al。，2003; Hugel和Schlichter，2000）。在这里，我们进行了一些修改，包括试剂，食谱，解剖和描述从新生小鼠的初步SDHN的解剖和培养的分步程序。在该方案中，使用来自新鲜脊髓背角组织的酶（木瓜蛋白酶）消化方法直接获得神经元。体外SDHN的培养可以用于进一步的实验，例如电生理记录，免疫细胞化学和Ca 2+成像，其更好地支持脊髓中的细胞行为。
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| Monocular Deprivation in Mice
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Author:
Date:
2014-01-20
[Abstract] Monocular deprivation is an experimental technique to study the ocular dominance plasticity during critical period (Hubel and Wiesel, 1963). Generally one eye of an animal is sutured during critical period, and the sutured eye is re-opened after either less than three days (short term) or more than three days (long term). Here we describe a detailed protocol for short-term and long-term monocular deprivation in mouse (Ma et al., 2013).
[摘要] 单眼剥夺是研究关键期眼部优势可塑性的实验技术（Hubel和Wiesel，1963）。 通常在关键时期缝合一只动物的眼睛，缝合眼睛在短短三天（短期）或超过三天（长期）之后重新开放。 这里我们描述了一个关于小鼠短期和长期单眼剥夺的详细方案（Ma et al。，2013）。
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