| Mouse Subependymal Zone Explants Cultured on Primary Astrocytes
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Author:
Date:
2016-07-20
[Abstract] Neuroblast migration is a major component in the process of neuronal circuit assembly. In the rodent postnatal brain, the subependymal zone (SEZ) is the largest neurogenic niche where new neurons are born throughout life. These cells migrate several millimeters following a specific pathway called the rostral migratory stream (RMS) towards the olfactory bulb. Once they reach their final destination, they differentiate mainly as interneurons and integrate into already established neuronal circuits. Along the RMS, neuroblasts use a particular mode of migration known as chain migration. They stay attached to each other while migrating and are intimately associated with adjacent astrocytic processes. To dissect the molecular and cellular signals involved in neuroblast migration, we developed ...
[摘要] 神经母细胞迁移是神经元电路组装过程中的主要组成部分。 在啮齿动物出生后的大脑中，子宫颈区（SEZ）是新生神经元生命中最大的神经源性生态位。 这些细胞沿着称为传播流（RMS）的特定途径朝向嗅球迁移几毫米。 一旦达到其最终目的地，它们主要区分为中间神经元并整合到已建立的神经元回路中。 沿着RMS，神经母细胞使用称为链转移的特定迁移模式。 它们在迁移时保持相互依赖，并与相邻的星形细胞过程密切相关。 为了解剖神经母细胞迁移中涉及的分子和细胞信号，我们开发了一种体外系统，其由在星形胶质细胞单层顶部共培养的经SEZ衍生的外植体组成。
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| Quantification of HIV RNA and Human Herpesvirus DNA in Seminal Plasma
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Author:
Date:
2015-05-05
[Abstract] Multiple viruses can co-infect the genital tract, modifying the immunologic and virologic milieu and possibly playing a role in viral transmission and pathogenesis. The aim of our studies has been to understand the complex relationships between HIV-1 RNA, and multiple human herpesviruses known to frequently replicate in the genital tract of HIV-infected men (i.e. cytomegalovirus [CMV], Epstein Bar virus [EBV], herpes simplex virus [HSV] types 1 and 2, and human herpesviruses [HHV] 6, 7 and 8) (Gianella et al., 2013a; Gianella et al., 2013b; Gianella et al., 2013c; Gianella et al., 2014). This protocol was designed to collect and process male genital secretion (GS), and to isolate and further quantify HIV RNA and DNA of seven HHV from seminal ...
[摘要] 多种病毒可以共感染生殖道，修改免疫学和病毒学环境，并可能在病毒传播和发病中起作用。 我们的研究的目的是了解HIV-1 RNA和已知在HIV感染的男性生殖道中经常复制的多种人疱疹病毒之间的复杂关系（即巨细胞病毒[CMV]，Epstein 条形病毒[EBV]，单纯疱疹病毒[HSV] 1型和2型，以及人疱疹病毒[HHV] 6,7和8）（Gianella等人，2013a; Gianella等人 。，2013b; Gianella 等人，2013c; Gianella ，2014）。 该方案设计用于收集和处理男性生殖器分泌（GS），并使用定量实时PCR技术从精浆中分离并进一步定量7个HHV的HIV RNA和DNA。
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| PBMC-MSC Co-cultures for Induction of Treg Generation
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Author:
Date:
2015-01-20
[Abstract] To assess the capacity of multipotent stromal cells (MSC) to induce the generation of Tregs, transwell co-cultures were performed as well as cultures with MSC-conditioned medium (CM). In short, peripheral blood mononuclear cells (PBMC) were co-cultured with allogeneic MSC or CM for one week followed by one week of culture in the absence of MSC.
[摘要] 为了评估多潜能基质细胞（MSC）诱导Treg产生的能力，进行Transwell共培养以及具有MSC条件培养基（CM）的培养。 简而言之，将外周血单核细胞（PBMC）与同种异体MSC或CM共培养一周，然后在不存在MSC的情况下培养一周。
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