|
Author:
Date:
2016-07-05
[Abstract] Macromolecules, proteins, lipids, and other small molecules, such as carotenoids can be studied within different tissues and organelles using an array of in vitro and in vivo methodologies. In the case of tomato and other fleshy fruit the predominant organelle in ripe fruit is the chromoplast. The characteristic feature of this organelle is the presence of pigments, carotenoids at high levels. In order to fully understand the underlying biological mechanisms that operate within the chromoplast, it is necessary to perform studies at the subchromoplast level. This protocol allows the separation of plastoglobules (lipoprotein particles, which are coupled to thylakoid membranes in the chloroplasts) and membranes (thylakoid, envelope-like) of chromoplasts through a sucrose ...
[摘要] 可以使用体外和体内方法的阵列在不同的组织和细胞器中研究大分子，蛋白质，脂质和其它小分子，例如类胡萝卜素。在番茄和其他肉果的情况下，成熟果实中的主要细胞器是色质体。这种细胞器的特征是存在高水平的颜料，类胡萝卜素。为了完全理解在chromoplast内操作的基本生物机制，有必要在subchromoplast水平进行研究。该方案允许通过蔗糖梯度分离塑料球（脂蛋白颗粒，其与叶绿体中的类囊体膜偶联）和膜（类囊体，包膜样）的色原体。然后可以独立地分析亚氯化银色室。突变体/转基因基因型与其背景之间的比较可以在同一分馏运行期间同时进行精确进行。该程序最初是为了成熟番茄果实开发的，但已经显示翻译成甜和辣椒。
|