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Potassium chloride

氯化钾

Company: Sigma-Aldrich
Catalog#: P5405
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Measurement of Dopamine Using Fast Scan Cyclic Voltammetry in Rodent Brain Slices
Author:
Date:
2018-10-05
[Abstract]  Fast scan cyclic voltammetry (FSCV) is an electrochemical technique that allows sub-second detection of oxidizable chemical species, including monoamine neurotransmitters such as dopamine, norepinephrine, and serotonin. This technique has been used to record the physiological dynamics of these neurotransmitters in brain tissue, including their rates of release and reuptake as well as the activity of neuromodulators that regulate such processes. This protocol will focus on the use of ex vivo FSCV for the detection of dopamine within the nucleus accumbens in slices obtained from rodents. We have included all necessary materials, reagents, recipes, procedures, and analyses in order to successfully perform this technique in the laboratory setting. Additionally, we have also included ... [摘要]  快速扫描循环伏安法(FSCV)是一种电化学技术,可以亚秒检测可氧化的化学物质,包括单胺类神经递质,如多巴胺,去甲肾上腺素和血清素。 该技术已被用于记录这些神经递质在脑组织中的生理动态,包括它们的释放和再摄取速率以及调节这些过程的神经调节剂的活性。 该方案将集中于使用离体 FSCV检测从啮齿动物获得的切片中的伏隔核内的多巴胺。 我们已经包含了所有必要的材料,试剂,配方,程序和分析,以便在实验室环境中成功地执行该技术。 此外,我们还提供了一些警示点,我们认为这些警告点对于那些在该领域的新手有帮助。

【背景】由于首先将用于检查生理系统的电特性的能力用于临床前科学研究,因此已经开发了许多用于研究突触生理学的技术。从鱿鱼轴突电生理记录的早期到现在的快速扫描循环伏安法(FSCV)在人类帕金森病患者中进行(Kishida et al。,2016; Lohrenz et al。,2016),该领域在相对较短的时间内取得了重大进展。该协议的重点是FSCV,是物理学家,分析化学家和神经科学家之间40多年的创新和合作的技术成果。早在19世纪(Bard和Zoski,2000年),电化学与Michael Faraday和Alessandro Volta一起诞生(Bard and Zoski,2000),直到20世纪20年代,Jaroslav ...

Qualitative in vivo Bioluminescence Imaging
Author:
Date:
2018-09-20
[Abstract]  Bioluminescence imaging (BLI) technology is an advanced method of carrying out molecular imaging on live laboratory animals in vivo. This powerful technique is widely-used in studying a variety of biological processes, and it has been an ideal tool in exploring tumor growth and metastatic spread in real-time. This technique ensures the optimal use of laboratory animal resources, particularly the ethical principle of reduction in animal use, given its non-invasive nature, ensuring that ongoing biological processes can be studied over time in the same animal, without the need to euthanize groups of mice at specific time points. In this protocol, the luciferase imaging technique was developed to study the effect of co-inoculating pericytes (contractile, αSMA+ mesenchymal ... [摘要]  生物发光成像(BLI)技术是一种在体内实验室动物上进行分子成像的先进方法。 这种强大的技术广泛应用于研究各种生物过程,是实时探索肿瘤生长和转移扩散的理想工具。 该技术确保实验室动物资源的最佳利用,特别是减少动物使用的伦理原则,考虑到其非侵入性,确保可以在同一动物中随时间研究正在进行的生物过程,而无需安乐死 小鼠在特定的时间点。 在该方案中,开发了荧光素酶成像技术以研究共同接种周细胞(收缩性,αSMA + 间充质干细胞样细胞,位于微血管内的细胞)对生长和转移性扩散的影响。 卵巢癌使用侵袭性卵巢癌细胞系-OVCAR-5-作为例子。

【背景】生物发光成像(BLI)的原理是基于相对简单的生化过程的发光特性,即,荧光素酶介导的分子底物荧光素氧化产生光。在癌症研究中,BLI是一种流行的工具(Contag et ...

Striatal Synaptosomes Preparation from Mouse Brain
Author:
Date:
2018-09-05
[Abstract]  The striatum is located in the subcortical region of the forebrain, it contains medium spiny neurons, cholinergic interneurons and GABAergic interneurons, and receives dopaminergic projection in the nigrostriatal pathway. This protocol provides a method to collect synaptosomes from mouse brain dorsal stratum. The synaptosomes can be used to study dopamine uptake activity, dopaminergic terminal endocytosis/trafficking using biochemical and microscopy methods, and protein analysis (Sorkina et al., 2018). [摘要]  纹状体位于前脑的皮质下区域,它包含中等刺状神经元,胆碱能中间神经元和GABA能中间神经元,并在黑质纹状体通路中接受多巴胺能投射。 该方案提供了从小鼠脑背侧层收集突触体的方法。 突触体可用于研究多巴胺摄取活性,使用生物化学和显微镜方法的多巴胺能末端内吞作用/运输,以及蛋白质分析(Sorkina 等,,2018)。

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