|
Author:
Date:
2013-12-20
[Abstract] During viral infection to host cells, several viruses undergo the process of endocytosis and pH-dependent fusion. By fusion of viral membrane with host cellular membrane, the viral core invades to host cytoplasm. A part of monoclonal antibodies against viral membrane protein have potential to inhibit the viral fusion step. Here we describe in vitro influenza virus-cell fusion inhibition assay. The infected cells expressing viral membrane protein, such as hemagglutinin (HA), on cellular surface are incubated with monoclonal antibodies targeting viral membrane protein. Then the cells are incubated under low pH condition. If the antibody does not inhibit the fusion step, we can see multinucleated giant cells.
[摘要] 在病毒感染宿主细胞期间，几种病毒经历内吞作用和pH依赖性融合的过程。 通过病毒膜与宿主细胞膜的融合，病毒核心侵入宿主细胞质。 抗病毒膜蛋白的单克隆抗体的一部分具有抑制病毒融合步骤的潜力。 在这里我们描述了体外流感病毒 - 细胞融合抑制试验。 将表达细胞表面上的病毒膜蛋白（例如血凝素（HA））的感染细胞与靶向病毒膜蛋白的单克隆抗体温育。 然后将细胞在低pH条件下温育。 如果抗体不抑制融合步骤，我们可以看到多核巨细胞。
|