| Isolation of Primary Breast Cancer Cells from HER2 Transgenic Mice
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Author:
Date:
2016-10-05
[Abstract] HER2 is a tyrosine kinase receptor, which is overexpressed in about 30% of breast cancer patients. Its overexpression leads to mammary tumorigenesis and increased invasion and metastasis (Slamon et al., 1987). HER2 transgenic mouse (FVB/N-MMTVneu mouse) is a well-established model of mammary tumor in human (Fantozzi and Christofori, 2006). Although in vivo models are excellent for assessing the influence of various factors, especially microenvironment, on development of breast cancer, a convenient and less costly way to study the underlying molecular events is utilizing cells derived from the model under evaluation. In order to explore the molecular mechanism by which HOXB7 inhibits initiation, but promotes metastasis of breast tumors, we generated mouse breast cancer ...
[摘要] HER2是酪氨酸激酶受体，其在约30％的乳腺癌患者中过表达。 其过表达导致乳腺肿瘤发生和增加的侵袭和转移（Slamon等人，1987）。 HER2转基因小鼠（FVB/N-MMTVneu小鼠）是人类乳腺肿瘤的良好建立的模型（Fantozzi和Christofori，2006）。 虽然体内模型对于评估各种因素，特别是微环境对乳腺癌发展的影响是极好的，但是一种方便且成本较低的研究基础分子事件的方法是利用来自模型的细胞 评价。 为了探索HOXB7抑制起始，但促进乳腺肿瘤转移的分子机制，我们从HER2转基因小鼠产生小鼠乳腺癌细胞系（Liu等人，2015）。 该方案可用于从具有其他遗传背景的小鼠产生乳腺癌细胞系。
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| Isolation and Culture of Neurospheres for the Study of Pathogenesis of Prion Disease
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Author:
Date:
2014-03-20
[Abstract] Neurosphere contains neural stem cells that are capable of self-renewal and multilineage differentiation including neurons, astrocytes, and oligodendrocytes (Gage, 2000). Cell culture model using differentiated neurosphere cultures are suggested to be a valuable tool for studying the pathogenesis of prion disease at the cellular level (Iwamaru et al., 2013). This protocol describes the procedure for a culture of whole brain-derived neurospheres from newborn mouse brains. Neurosphere formation steadily occurs within a week from the cultures of neonatal whole brains and these cells have stem cell properties.
[摘要] 神经球包含能够自我更新和多向分化的神经干细胞，包括神经元，星形胶质细胞和少突胶质细胞（Gage，2000）。 使用分化的神经球培养物的细胞培养模型被认为是用于研究朊病毒疾病在细胞水平的发病机制的有价值的工具（Iwamaru等人，2013）。 该协议描述了来自新生小鼠脑的全脑衍生的神经球的培养的程序。 神经球形成稳定地发生在新生儿全脑的培养物的一周内，并且这些细胞具有干细胞特性。
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| Neural Stem Cell Differentiation and Prion Infection
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Author:
Date:
2014-03-20
[Abstract] Prion diseases are transmissible, fatal, neurodegenerative diseases in human and animals. The molecular basis of neurodegeneration in prion diseases is largely unclear. Developing a cellular model capable of monitoring prion-induced cytotoxicity would be a promising approach for better understanding the prion pathogenesis. One candidate cellular assay is a model based on neurospheres, which contains neural stem cells (NSCs). Both undifferentiated and differentiated NSCs have been demonstrated to be permissive to prion infection, and prion-induced cytopathic changes in differentiated neruosphere cultures were reported (Iwamaru et al., 2013). This protocol describes the procedure to induce differentiation of NSCs from transgenic mice overexpressing prion protein (tga20 mice) into ...
[摘要] 朊病毒疾病是人类和动物中可传播的，致命的，神经变性疾病。 朊病毒疾病中神经变性的分子基础很大程度上不清楚。 开发能够监测朊病毒诱导的细胞毒性的细胞模型将是更好地理解朊病毒发病机制的有前途的方法。 一种候选细胞测定是基于神经球的模型，其含有神经干细胞（NSC）。 未分化和分化的NSCs都被证明是容许朊病毒感染，并且报道了分化的神经球培养物中的朊病毒诱导的细胞病变变化（Iwamaru等人，2013）。 该协议描述了诱导NSCs从过度表达朊病毒蛋白（tga20小鼠）的转基因小鼠分化成易感染朊病毒感染的培养物的过程。
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