Student Vannas Spring Scissors, Straight
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Functional ex-vivo Imaging of Arterial Cellular Recruitment and Lipid Extravasation
Author:
Date:
2017-06-20
[Abstract]  The main purpose of this sophisticated and highly versatile method is to visualize and quantify structural vessel wall properties, cellular recruitment, and lipid/dextran extravasation under physiological conditions in living arteries. This will be of interest for a broad range of researchers within the field of inflammation, hypertension, atherosclerosis, and even the pharmaceutical industry. Currently, many researchers are using in vitro techniques to evaluate cellular recruitment, ...

Microinjection of Virus into Lumbar Enlargement of Spinal Dorsal Horn in Mice
Author:
Date:
2016-11-20
[Abstract]  In order to explore the role of a specific gene/protein in the specific segment of the spinal cord, the technique of intraspinal injection is particularly used to deliver viral vectors targeting the specific gene/protein. These viral vectors can knockdown or overexpress the specific gene/protein in specific cells (glial cells or neurons). In this protocol, lentivirus containing shRNA for CXCL13 were injected into the dorsal horn of the spinal lumbar enlargement segment (Jiang et al., ...

Isolation and Primary Cell Culture of Mouse Dorsal Root Ganglion Neurons
Author:
Date:
2016-04-05
[Abstract]  We here provide a detailed protocol for the isolation and culture of primary mouse sensory neurons. The cell bodies of sensory afferent pseudounipolar neurons are located in dorsal root ganglia (DRGs) along the vertebral column. Dissected mouse DRGs can be dissociated into single cells by enzymatic digestion to obtain primary cultures of mouse sensory neurons as performed in the studies reported by Khaminets et al. (2015).

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