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Author:
Date:
2016-03-20
[Abstract] The auditory brainstem response (ABR) test provides information about the inner ear (cochlea) and the central pathways for hearing. The ABR reflects the electrical responses of both the cochlear ganglion neurons and the nuclei of the central auditory pathway to sound stimulation (Zhou et al., 2006; Burkard et al., 2007). The ABR contains 5 identifiable wave forms, labeled as I-V. Wave I represents the summated response from the spiral ganglion and auditory nerve while waves II-V represent responses from the ascending auditory pathway. The ABR is recorded via electrodes placed on the scalp of an anesthetized animal. ABR thresholds refer to the lowest sound pressure level (SPL) that can generate identifiable electrical response waves.
This protocol describes ...
[摘要] 听觉脑干反应（ABR）测试提供有关内耳（耳蜗）和听觉中枢通路的信息。 ABR反映了耳蜗神经节神经元和中枢听觉通路的核对声音刺激的电响应（Zhou et al。，2006; Burkard et al。，2007）。 ABR包含5个可识别的波形，标记为I-V。波I代表螺旋神经节和听觉神经的总和反应，而波II-V代表上升听觉通路的反应。通过置于麻醉动物头皮上的电极记录ABR。 ABR阈值是指可以产生可识别的电响应波的最低声压级（SPL）。
该协议描述了测量小型啮齿动物（小鼠，大鼠，豚鼠等）的ABR的过程，包括麻醉鼠标，将电极放在头皮上，记录点击和色调脉冲串刺激，并读取获得的ABR阈值波形值。随着技术的不断发展，ABR可能提供更多关于听觉神经和脑干途径功能的定性和定量信息。
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