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Bovine Serum Albumin

牛血清白蛋白

Company: Sigma-Aldrich
Catalog#: A8022
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Using xCELLigence RTCA Instrument to Measure Cell Adhesion
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Date:
2017-12-20
[Abstract]  Cell adhesion to neighbouring cells and to the underlying extracellular matrix (ECM) is a fundamental requirement for the existence of multicellular organisms. As such, the formation, stability and dissociation of cell adhesions are subject to tight control in space and time and perturbations within the sophisticated adhesion machinery are associated with a variety of human pathologies. Here, we outline a simple protocol to monitor alterations in cell adhesion to the ECM, for example, following genetic manipulations or overexpression of a protein of interest or in response to drug treatment, using the xCELLigence real-time cell analysis (RTCA) system. [摘要]  细胞与相邻细胞和基础细胞外基质(ECM)的粘附是多细胞生物存在的基本要求。 因此,细胞粘附的形成,稳定和解离在时间和空间上受到严格的控制,复杂的粘附机制内的干扰与各种人类病理有关。 在这里,我们概述了一个简单的协议,以监测细胞粘附到ECM的变化,例如,在遗传操作或目标蛋白的过表达或响应药物治疗后,使用xCELLigence实时细胞分析(RTCA)系统。

【背景】负责细胞粘附到下面的ECM的主要分子是称为整联蛋白的跨膜异二聚体受体家族。整合素的激活和与ECM的结合触发向整合素胞质尾部募集大量信号传导,支架和细胞骨架蛋白。总之,这些粘附成分代表了负责调节许多重要细胞过程(包括细胞增殖,存活,迁移和分化)的复杂且高度动态的机制。与维持正常生理功能的重要角色一致,整合素介导的粘附和信号传导失调是许多人类疾病(包括出血性疾病,心血管疾病和癌症)发病的先导(Giancotti and Ruoslahti,1999;Bökeland Brown,2002; Huveneers and Danen,2009; Legate等人,2009; Bouvard等人,2013; Calderwood等人,2013; Horton等人,等,2015; Seguin等,,2015)。因此,整合素依赖性细胞 - 细胞外基质粘附的研究是一个研究热点,也是许多生物学领域广泛关注的课题。 ...

Dissection and Staining of Mouse Brain Ventricular Wall for the Analysis of Ependymal Cell Cilia Organization
Author:
Date:
2016-03-20
[Abstract]  In the developing and mature central nervous system (CNS) the ventricular lumen is lined by the neuroepithelium and ependymal, respectively. These ventricular epithelia perform important functions related to the development, morphogenesis and physiology of the brain. In the mature CNS, ependyma constitutes a barrier between brain parenchyma and cerebro- spinal fluid (CSF). The most prominent feature of the apical surface of ependymal cells is the presence of multiple motile cilia that extend towards the ventricular lumen. The beating of cilia ensures the circulation of the CSF and its impairment leads to hydrocephalus. For an effective CSF flow, ciliary beating must be coordinated at the level of individual cells and at the tissue level. This coordination is achieved through the precise ... [摘要]  在发展中和成熟的中枢神经系统(CNS)中,心室腔分别由神经上皮和室管膜排列。这些心室上皮执行与脑的发育,形态发生和生理相关的重要功能。在成熟CNS中,室管膜构成脑实质和脑脊液(CSF)之间的屏障。室管膜细胞的顶面的最突出的特征是存在向心室腔延伸的多个运动性纤毛。纤毛的跳动确保CSF的循环,并且其损伤导致脑积水。对于有效的CSF流动,睫状细胞跳动必须在单个细胞水平和组织水平协调。这种协调是通过精确组织在室内平面内的纤毛定位来实现的。已经描述了关于室管膜细胞中纤毛的平面组织的两个主要特征(Mirzadeh等人,2010),并且它们都具有细胞和组织方面(Boutin等人 ,2014)。第一个,旋转极性,指睫毛跳动的方向。在细胞水平,所有纤毛在相同方向跳动(图1B,黑色箭头)。在组织水平,每个室管膜细胞协调其拍打的方向与相邻细胞的方向(图1C,灰色箭头)。第二个特征,翻译极性,对室管膜细胞是唯一的,是指簇中的纤毛聚集。在细胞水平,该簇相对于室管膜细胞的中心位移(图1B,红色箭头)。在组织水平,睫状簇的定位在相邻细胞之间协调(图1C)。在任一水平上改变任何这些极性都会损害CSF流动循环(Mirzadeh等人,2010; Boutin等人,2014; Guirao等人,/em,2010; Hirota等人,2010; ...

In vitro Differentiation of Murine Innate Lymphoid Cells from Common Lymphoid Progenitor Cells
Author:
Date:
2016-03-20
[Abstract]  Subtypes of innate lymphoid cells (ILC), defined based on their cytokine secretion profiles and transcription factor expression, are important for host protection from pathogens and maintaining tissue homeostasis. ILCs develop from common lymphoid progenitors (CLP) in the bone marrow. Using the methods described here, we have previously shown that loss of the transcriptional regulator TOX (Thymocyte-selection associated HMG-box protein) leads to specific changes in ILC development and differentiation. Here, we describe how to obtain ILCs from in vivo isolated CLP grown in vitro. [摘要]  先天淋巴细胞(ILC)的亚型,基于其细胞因子分泌谱和转录因子表达来定义,对于宿主保护免于病原体和维持组织内稳态是重要的。 ILC从骨髓中的普通淋巴祖细胞(CLP)发展而来。 使用本文所述的方法,我们以前显示转录调节器TOX(胸腺细胞选择相关的HMG框蛋白)的损失导致ILC发展和分化的具体变化。 在这里,我们描述了如何从体内分离的CLP获得ILC。

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