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Omnifix®-F Duo, 25 G, Außen-ø: 0,5 mm, Länge: 16 mm

Omnifix -F Duo，25G，Außen-ø：0.5mm，长度：16mm
Company: B. Braun Medical
Catalog#: 9161465V
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Mouse Oocyte Isolation, Cultivation and RNA Microinjection
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Date:
2016-02-05
[Abstract]   Mammalian oocyte is a highly specialized cell, characterized by synthesis and storage of maternal proteins and RNAs that contributes to the meiotic cell cycle and early embryo development. The fully grown oocyte is transcriptionally quiescent and utilizes only transcripts synthesized and stored during the growing phase. Mouse oocytes are often used as a mammalian model for the study of molecular biology of the cell or biomedical research. Microinjection technique is a useful tool to deliver RNA coding for fluorescently tagged proteins to determine their subcellular localization or function, delivering biosensors for the study of various metabolic pathways or downregulation of specific targets by RNAi or oligo morpholinos to study gene function. Here, we describe a protocol for isolation, ... [摘要]  哺乳动物卵母细胞是高度特化的细胞，其特征在于合成和储存母体蛋白质和RNA，其有助于减数分裂细胞周期和早期胚胎发育。 完全生长的卵母细胞是转录性静止的，并且仅利用在生长期期间合成和储存的转录物。 小鼠卵母细胞通常用作哺乳动物模型用于研究细胞的分子生物学或生物医学研究。 显微注射技术是递送编码荧光标记蛋白的RNA以确定其亚细胞定位或功能，递送生物传感器用于研究各种代谢途径或通过RNAi或寡聚morpholinos下调特定靶标以研究基因功能的有用工具。 在这里，我们描述了可能有助于研究或教育目的的卵母细胞的分离，培养和显微注射的协议。

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