Author:
Date:
2021-02-20
[Abstract] Current methods to obtain mesenchymal stem cells (MSCs) involve sampling, culturing, and expanding of primary MSCs from adipose, bone marrow, and umbilical cord tissues. However, the drawbacks are the limited numbers of total cells in MSC pools, and their decaying stemness during in vitro expansion. As an alternative resource, recent ceiling culture methods allow the generation of dedifferentiated fat cells (DFATs) from mature adipocytes. Nevertheless, this process of spontaneous dedifferentiation of mature adipocytes is laborious and time-consuming. This paper describes a modified protocol for in vitro dedifferentiation of adipocytes by employing an additional physical stimulation, which takes advantage of augmenting the stemness-related Wnt/β-catenin signaling. Specifically, this ...
[摘要] [摘要]目前的方法,以获得间充质干细胞(MSC)包括采样,培养,和扩大主要由脂肪,骨髓,和脐带组织的MSCs。然而,缺点是在总细胞在MSC池,和它们的衰减干性的数量有限在维生素- [R Ò扩张。作为替代资源,最近的天花板培养方法允许从成熟的脂肪细胞中生成去分化的脂肪细胞(DFAT)。然而,这种成熟脂肪细胞自发去分化的过程既费力又费时。本文描述了一种用于经修改协议在体外通过采用附加的物理刺激,其中脂肪细胞去分化TA KES扩充所述干性相关的优点的Wnt /β-catenin信号。具体来说,该协议利用含聚乙二醇(PEG)的高渗介质引入细胞外物理刺激以获得更高的效率,并引入更简单的脂肪细胞去分化程序。
[背景]脂肪组织由于其丰度大且侵袭性相对较低,因此是间充质干细胞(MSC)最具吸引力的来源之一(Shen等,2011 ;González-Cruz等,2012; Konno等人,2013)。脂肪来源的MSC,即从皮下脂肪组织的基质血管级分中分离,已被证实同时显示多谱系潜能的体外和体内(Anghileri等人,2008;冈萨雷斯。等人,2009;冈萨雷斯-雷伊等等人,2010; Jumabay等人,2010; Mao等人,2017和2019 ;Darnell等人,2018 ...
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Author:
Date:
2016-02-05
[Abstract] In recent years, the utilization of stem cell therapy to regenerate cardiac tissue has been proposed as a possible strategy to treat cardiac damage (Gnecchi et al., 2012, Aguirre et al., 2013; Sanganalmath and Bolli, 2013). Although encouraging results have been obtained in experimental models, the efficiency of cardiac regeneration is very poor and one of the major barriers to progress in the area of cell therapy for damaged heart is represented by the limited capacity of cells to differentiate into mature cardiomyocytes (CMC) (Laflamme and Murry, 2011). Cell manipulation and transfection represent versatile tools in this context (Melo et al., 2005; Dzau et al., 2005). Murine P19 embryonal carcinoma cells are a well-established cell line capable of ...
[摘要] 近年来,已经提出利用干细胞治疗来再生心脏组织作为治疗心脏损伤的可能策略(Gnecchi等人,2012,Aguirre等人, >,2013; Sanganalmath和Bolli,2013)。虽然在实验模型中已经获得令人鼓舞的结果,但是心脏再生的效率非常差,并且在损伤的心脏的细胞治疗领域中进展的主要障碍之一是细胞分化成成熟心肌细胞的能力有限(CMC )(Laflamme和Murry,2011)。细胞操作和转染在本文中代表多种工具(Melo等人,2005; Dzau等人,2005)。鼠P19胚胎癌细胞是能够在体外分化成自发性搏动CMC的良好建立的细胞系。这种细胞系统具有有限的细胞培养要求,协议重现性和易于摄取和随后的异位遗传材料的表达,使其理想的心脏分化过程的研究。 P19细胞已经成功地用于获得对CMC分化的早期分子过程的重要见解(van der Heyden和Defize,2003; van der ...
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