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Author:
Date:
2016-02-05
[Abstract] Lipid-Protein interaction assay is a method to search lipids, which are bound with proteins in vitro. Since membranes that are spotted with chloroplast lipids such as monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), and sulfoquinovosyldiacylglycerol (SQDG) are not commercially available, we extracted these lipids from cyanobacterial cells and spotted them onto membranes. The prepared membranes could be used for lipid-protein interaction assay.
[摘要] 脂质 - 蛋白质相互作用测定法是一种搜索在体外与蛋白质结合的脂质的方法。 由于用叶绿体脂质例如单半乳糖基二酰基甘油（MGDG），二半乳糖基二酰基甘油（DGDG）和磺基奎诺糖基二酰基甘油（SQDG）点样的膜不可商购，因此我们从蓝藻细胞中提取这些脂质并将它们点样到膜上。 所制备的膜可用于脂质 - 蛋白质相互作用测定。
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