| Batch Culture Fermentation of Endophytic Fungi and Extraction of Their Metabolites
|
|
Author:
Date:
2013-10-05
[Abstract] Antibiosis is one of the possible modes of action shown by endophytic fungi having antifungal activity. To test if antifungal activity in endophytic fungi is due to antibiosis, assay of the metabolites of endophytic fungi was needed. To obtain metabolites for bioassay batch culture fermentation and extraction of metabolites was done. Fungus was multiplied on wickerham media at incubation temperature of 25 ± 2 °C for 4 weeks and then extracted with solvents of different polarity. All the solvent extracts were dried under vacuum rotary evaporator to get dried crude fungal extract, which was subjected to further fractionation and bioassay.
[摘要] 抗生素是具有抗真菌活性的内生真菌所显示的可能的作用模式之一。 为了测试内生真菌中的抗真菌活性是否是由于抗生素，需要测定内生真菌的代谢物。 为了获得用于生物测定的代谢物，进行批次培养发酵和代谢物的提取。 将真菌在wickerham培养基上在25±2℃的孵育温度下繁殖4周，然后用不同极性的溶剂萃取。 所有溶剂提取物在真空旋转蒸发器下干燥，得到干燥的粗真菌提取物，对其进行进一步分馏和生物测定。
|
|
|
| Bioassay of Extracts of the Endophytic Fungi
|
|
Author:
Date:
2013-10-05
[Abstract] Many of the microbes including fungi produce metabolites possessing antifungal activity and in some cases fungal metabolites are main cause of antifungal activity resulted by fungus. To infer that antifungal activity is due to fungal metabolites, there is a need to develop a repeatable procedure to assay these metabolites. Here we are presenting the poisoned food technique for bioassay of extract from endophytic fungi, where culture media is supplemented with extract to testpathogen viability as proxy for antifungal activity.
[摘要] 许多微生物包括真菌产生具有抗真菌活性的代谢物，在一些情况下，真菌代谢物是真菌导致的抗真菌活性的主要原因。 为了推断抗真菌活性是由于真菌代谢物，需要开发一种可重复的方法来测定这些代谢物。 在这里，我们提出的中毒食品技术生物测定的内生真菌提取物，其中培养基添加提取物作为抗真菌活性的代理试验病原体的生存力。
|
|
|