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4,5-Diaminofluorescein diacetate

4,5-二氨基荧光素二乙酸酯

Company: Sigma-Aldrich
Catalog#: D2813
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Measurement of Endogenous H2O2 and NO and Cell Viability by Confocal Laser Scanning Microscopy
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2013-10-05
[Abstract]  Recently, there is compelling evidence that hydrogen peroxide (H2O2) and nitric oxide (NO) function as signaling molecules in plants, mediating a range of responses including stomatal movement. Thus, the choice of sensitive methods for detection of endogenous H2O2 and NO in guard cells are very important for understanding the role of H2O2 and NO in guard cell signaling. In addition, besides stomatal closure caused by interfering guard cell signaling, it can also be caused by widespread, nonspecific damage to guard cells. To determine whether stomatal movement is caused by damage to guard cells, sensitive methods for detection of guard cell viability are often required.

The oxidatively sensitive fluorophore ...
[摘要]  最近,有令人信服的证据表明,过氧化氢(H 2 O 2 O 2)和一氧化氮(NO)作为植物中的信号分子起作用,介导一系列反应,包括气孔运动。因此,选择用于检测保卫细胞中内源性H 2 O 2 O 2和NO的灵敏方法对于理解H 2 sub的作用是非常重要的> O 2和保守细胞信号中的NO。此外,除了由干扰保卫细胞信号传导引起的气孔闭合之外,它还可以由保护细胞的广泛的非特异性损伤引起。为了确定气孔运动是否由保卫细胞的损伤引起,通常需要用于检测保卫细胞存活力的灵敏方法。
氧化敏感荧光团2',7'-二氯荧光素(H 2 DCF)通常用于测量细胞内H 2 O 2 O 2的变化,/sub>级别。 H 2 DCF的非极性二乙酸酯(H 2 DCFDA)进入细胞,并水解成极性更强的非荧光化合物H 2,/sub> DCF,因此,它被捕获。随后通过过氧化物酶催化的H 2 O 2 O 2对H 2 DCF的氧化产生高度荧光的DCF。类似地,细胞可渗透的NO-敏感性荧光探针4,5-二氨基荧光素二乙酸酯(DAF-2DA)广泛用于在动物和植物细胞中直接检测NO存在。非极性DAF-2DA进入细胞,并被胞质酯酶水解为极性更强的非荧光化合物DAF-2,其在NO存在下转化为高度荧光的三唑衍生物DAF-2T。荧光指示剂染料荧光素二乙酸酯(FAD)和碘化丙啶(PI)广泛用于检测细胞活力。 ...

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