|
Author:
Date:
2016-01-20
[Abstract] Human hepatic cancer cell lines such as HepG2, Huh7, and HLE cannot get infected with Hepatitis B virus (HBV) due to lack of an HBV receptor(s). Transfection with HBV genome has so far been referred as a tool to mimic HBV infection. However, since sodium taurocholate cotransporting polypeptide (NTCP) was identified as a functional receptor for HBV (Yan et al., 2012), hepatocyte cell lines that were stably transfected with a plasmid for NTCP expression have been used for HBV infection. This protocol is designed for infection with HBV in human hepatocyte cell line HepG2 expressing NTCP (HepG2-hNTCP-C4 cells; Iwamoto et al., 2014) or primary human hepatocytes (PHHs). In this section, we also describe one of the methods for the assessment of HBV infection: Quantification of ...
[摘要] 人肝癌细胞系如HepG2，Huh7和HLE由于缺乏HBV受体而不能感染乙型肝炎病毒（HBV）。 HBV基因组的转染迄今为止被称为模拟HBV感染的工具。 然而，由于牛磺胆酸钠共转运多肽（NTCP）被鉴定为HBV的功能性受体（Yan等人，2012），已经使用用用于NTCP表达的质粒稳定转染的肝细胞细胞系 为HBV感染。 该方案设计用于在表达人类肝细胞细胞系HepG2的表达NTCP（HepG2-hNTCP-C4细胞; Iwamoto等人，2014）或原代人肝细胞（PHH）的HBV感染。 在本节中，我们还描述了用于评估HBV感染的方法之一：细胞内衣壳化的HBV DNA的定量。
|