| Quantification of Low Molecular Weight Thiols in Arabidopsis
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Author:
Date:
2016-01-05
[Abstract] Low-molecular-weight (LMW) thiols are a class of highly reactive compounds due to their thiol moiety. They play important roles in the maintenance of cellular redox homeostasis, detoxification, and development. Monobromobimane (mBBr) is weakly fluorescent but selectively reacts with thiols to yield highly fluorescent thioethers (mBSR) products, which is especially useful for the quantification of LMW thiols. The stable mBSR products can be separated by high-performance liquid chromatography (HPLC) equipped with a fluorescent detector. The main cellular LMW thiols are L-cysteine, gamma-glutamylcysteine, and glutathione (GSH). The following protocol describes the extraction and quantification of L-cysteine, gamma-glutamylcysteine, and glutathione from Arabidopsis tissues as ...
[摘要] 低分子量（LMW）硫醇是一类高反应性化合物，由于它们的硫醇部分。 它们在维持细胞氧化还原稳态，解毒和发育中起重要作用。 单溴溴烷（mBBr）是弱荧光的，但选择性地与硫醇反应产生高荧光硫醚（mBSR）产物，其对于定量LMW硫醇特别有用。 稳定的mBSR产物可以通过配备有荧光检测器的高效液相色谱（HPLC）分离。 主要的细胞LMW硫醇是L-半胱氨酸，γ-谷氨酰半胱氨酸和谷胱甘肽（GSH）。 以下方案描述了从所报道的拟南芥组织中提取和定量L-半胱氨酸，γ-谷氨酰半胱氨酸和谷胱甘肽（Xiang和Oliver，1998; Zhao等人， 2014; Wang ，，2015）。 如果HPLC系统或C18柱不同，可能需要进行修改。
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| Measuring the Arsenic Content and Speciation in Different Rice Tissues
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Author:
Date:
2015-04-20
[Abstract] Arsenic (As) plays an important role in rice production as vast soils used for rice cultivation contain As. To understand how rice plants deal with inorganic As (III) and As (V) and organic As in their tissue, it is important to obtain specific information on how much and what species of As are present in which tissue of the rice plant. The protocol presented here allows to analyse the As contents and As speciation in roots, shoots, and husks of rice plants, and thus permits direct comparison of the As contents of these rice plant tissues.
[摘要] 砷（As）在稻米生产中起重要作用，因为用于稻米栽培的广大土壤含有As。 要了解水稻植物如何处理无机砷（III）和As（V）和有机砷在他们的组织，重要的是获得具体的信息，有多少和什么种类的As存在于水稻植物的组织。 这里介绍的协议允许分析水稻植物的根，芽和谷物中的As含量和As形态，因此允许直接比较这些水稻植物组织的As含量。
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