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Trypan Blue solution

台盼蓝溶液

Company: Sigma-Aldrich
Catalog#: T8154
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Generation of Human Mesenchymal Stem Cell 3D Spheroids Using Low-binding Plates
Author:
Date:
2018-08-20
[Abstract]  The 3D culture of human mesenchymal stem cells (hMSCs) represents a more physiological environment than classical 2D culture and has been used to enhance the MSC secretome or extend cell survival after transplantation. Here we describe a simple and affordable method to generate 3D spheroids of hMSCs by seeding them at high density in a low-binding 96-well plate.

Spheroids of hMSCs cultured in low-binding 96-well plates can be used to study the basic biology of the cells and to generate conditioned media or spheroids to be used in transplantation therapeutic approaches. These MSCs or their secretome can be used as a regenerative therapy and for tissue repair across multiple disease areas, including neurodegeneration.

In comparison to other methods (hanging drop, use ...
[摘要]  人间充质干细胞(hMSC)的3D培养代表比经典2D培养更生理的环境,并且已经用于增强MSC分泌组或移植后延长细胞存活。 在这里,我们描述了一种简单且经济实惠的方法,通过在低密度96孔板中高密度接种hMSC来生成三维球状体。

在低结合96孔板中培养的hMSC的球状体可用于研究细胞的基本生物学并产生用于移植治疗方法的条件培养基或球状体。 这些MSC或其分泌蛋白组可用作再生疗法和用于多个疾病区域的组织修复,包括神经变性。

与其他方法(悬滴,使用凝胶或生物材料,磁悬浮,等)相比,此处描述的方法简单且经济实惠,无需使用专用设备,昂贵材料或复杂试剂。

【背景】 间充质干细胞(MSCs)是开发针对中风或肌萎缩侧索硬化等疾病的新型再生疗法的有吸引力的候选者(Chen et al。,2001; Bang et al。,2005; Boido et al。,2014)。它们的多功能性使得技术的优化和标准化对于确保MSC疗法可以提供尽可能多的益处是必不可少的。使MSC的治疗潜力(例如,增强的抗炎介质分泌)最大化的一种可能方式是以3D方式培养它们(Bartosh ...

Isolation and Maintenance of Striatal Neurons
Author:
Date:
2018-04-20
[Abstract]  Primary cultures of murine striatal neurons are widely used to explore cellular mechanisms in neurobiology, including brain diseases. Here we describe a detailed and standardized protocol to dissect and culture embryonic murine striatal neurons GABA-positive/DARPP-32-positive for 12 days in vitro, when they show good neuronal cell connectivity and the presence of dendritic spines, which reflects the maturation of the network. [摘要]  小鼠纹状体神经元的原代培养物被广泛用于探索神经生物学中的细胞机制,包括脑部疾病。 在这里,我们描述了一个详细和标准化的协议解剖和文化胚胎小鼠纹状体神经元GABA阳性/ DARPP-32阳性12天体外,当他们显示良好的神经元细胞连接性和存在的树突 刺,反映了网络的成熟。

【背景】纹状体是运动和奖励系统的重要组成部分,纹状体神经元的功能障碍可导致各种神经元疾病,从强迫性行为(Welch et al。,2007)到神经退行性疾病,如在亨廷顿病中观察到的(Reiner等,1988)。因此,一个良好建立的纹状体文化可能是一个很有价值的研究这些和其他条件的模型。成年纹状体中的主要神经元亚型是中型多刺投射神经元(MSNs),其占所有纹状体神经元的约95%并使用抑制性递质γ-氨基丁酸(GABA)。它们具有中等大小的细胞体,复杂的树突状乔木和高密度的树突棘,可同时接受谷氨酸能和多巴胺输入。其余5%的神经元由GABA能神经间神经元组成(Graveland和DiFiglia,1985; ...

Isolation and Establishment of Mesenchymal Stem Cells from Wharton’s Jelly of Human Umbilical Cord
Author:
Date:
2018-02-20
[Abstract]  Mesenchymal stem cells (MSCs) are currently considered as ‘medicinal signaling cells’ and a promising resource in regard to cell-based regenerative therapy. Umbilical cord is a human term perinatal tissue which is easily attainable, and a promising source of stem cells with no associated ethical concerns. MSCs have been isolated from different regions of the umbilical cord and Wharton’s jelly (WJ) is the gelatinous matrix that surrounds and provides protection to the umbilical cord blood vessels. Being more primitive, MSCs from human umbilical cord exhibit greater proliferative capacity and immunosuppressive ability as compared to adult stem cells which gives them a therapeutic advantage. To meet the requirements for cell therapy, it is important to generate MSCs at a clinical scale by ... [摘要]  间充质干细胞(mesenchymal stem cells,MSCs)目前被认为是“医药信号传导细胞”,在基于细胞的再生治疗方面是一种很有前景的资源。脐带是人类的围产期组织,很容易实现,是一种有前途的干细胞来源,没有相关的伦理问题。 MSC已经从脐带的不同区域分离出来,而Wharton's果冻(WJ)是包围并提供对脐带血管的保护的凝胶状基质。更原始的是,与成人干细胞相比,来自人脐带的MSC表现出更大的增殖能力和免疫抑制能力,这使其具有治疗优势。为了满足细胞疗法的要求,通过遵循不耗时或劳动强度的步骤来产生临床规模的MSC是重要的。在此我们提出了一种简单,高效的方法,通过外植体培养方法从人脐带WJ中分离出MSC,这种方法具有重现性和成本效益。

【背景】间充质干细胞(MSC)具有显着的临床潜力来治疗各种衰弱性疾病,主要是由于其独特的免疫调节作用和再生能力(Caplan and Sorrell,2015)。它们存在于许多组织中(Hass et al。,2011),并被观察到是血管周围的体内(Caplan和Correa,2011)。来源或来源本身的小生境可能导致各种MSC类型之间的重要功能差异(Kwon等人,2016年)。虽然骨髓是研究得最充分和最好的MSCs来源,但也有一定的局限性(Liu et al。,2016)。 ...

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