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Author:
Date:
2016-01-05
[Abstract] 2-Methylthio-N6-isopentenyladenosine (ms2i6A) is an evolutionally conserved posttranscriptional modification found at position 37 of four mammalian mitochondrial tRNAs, mt-tRNASer(UCN), mt-tRNATrp, mt-tRNAPhe and mt-tRNATyr. The ms2 modification in ms2i6A strengthens codon-anticodon interaction and contributes to accurate and efficient decoding. Deficiency of ms2 modifications impairs mitochondrial protein synthesis, which ultimately leads to the development of myopathy in mice and patients having mitochondrial diseases. Therefore, the level of ms2 could be utilized as an indicator that reflects the status of mitochondrial protein synthesis. Here, we ...
[摘要] 2-甲硫基 - N 6 - 叔丁基腺苷（ms）是一种进化保守的转录后修饰发现 在四个哺乳动物线粒体tRNA，mt-tRNA Ser（UCN），mt-tRNA Trp ，mt-tRNA < trna=""> Tyr 。 ms 2 6中的ms 2修饰加强了密码子 - 反密码子相互作用，并有助于准确和有效的解码。 缺乏修复损害线粒体蛋白质合成，其最终导致小鼠和患有线粒体疾病的患者中的肌病的发展。 因此，ms 2的水平可以用作反映线粒体蛋白质合成状态的指标。 在这里，我们描述了一种简单和快速的定量PCR为基础的方法来测量总RNA样本中的ms 2水平。
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