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Author:
Date:
2015-10-05
[Abstract] Callose is an amorphous homopolymer, composed of β-1, 3-glucan, which is widespread in higher plants. Callose is involved in multiple aspects of plant growth and development. It is synthetized in plants at the cell plate during cytokinesis, in several stages during pollen development and is deposited at plasmodesmata to regulate the cell-to-cell movement of molecules. Moreover, it is produced in response to multiple biotic and abiotic stresses (Chen and Kim, 2009). Callose is considered to act as a physical barrier by strengthening the plant cell well to slow pathogen infection and to contribute to the plant’s innate immunity. Thus the callose staining method is useful to quantify activity of plant immunity. In addition, this staining can be used to visualize structures in plant tissue, ...
[摘要] Callose是由β-1,3-葡聚糖组成的无定形均聚物，其广泛存在于高等植物中。 Callose涉及植物生长和发育的多个方面。其在细胞分裂期间在细胞板处的植物中合成，在花粉发育期间的几个阶段中，并且沉积在浆细胞上以调节分子的细胞至细胞运动。此外，它是响应多种生物和非生物胁迫产生的（Chen和Kim，2009）。考虑通过加强植物细胞以缓慢病原体感染并有助于植物的先天免疫来考虑Callose作为物理屏障。因此，胼lose质染色方法可用于定量植物免疫的活性。此外，这种染色可用于显现植物组织中的结构，其中胼cal质可以暗示在植物发育期间或对病原体感染的反应。该方法基于使用与（1→3）-β-葡聚糖反应以在UV光中产生明亮的黄色荧光的甲基蓝。此外，calcofluor对存在于真菌细胞膜中的壳多糖染色，并且在角质层损伤的位置结合纤维素。
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