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Swinging Bucket, 6 x 5 mL, 55,000 rpm, 368,000 x g

摇动桶,6×5mL,55,000rpm,368,000×g

Company: Beckman Coulter
Catalog#: SW51Ti
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Preparation of Pre- and Post-synaptic Density Fraction from Mouse Cortex
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Date:
2013-09-05
[Abstract]  The understanding of the organization of postsynaptic signaling systems at excitatory synapses has been aided by the identification of proteins in the postsynaptic density (PSD) fraction, a subcellular fraction enriched in structures with the morphology of PSDs. Here we described an efficient way to isolate the crude synaptosome, presynaptic fraction, and PSD fraction. It helps to identify the location of synaptic protein and find the potential synaptic complex.
[摘要]  突触后信号系统在兴奋性突触的组织的理解已通过在突触后密度(PSD)部分,富含结构与PSD的形态的亚细胞部分中的蛋白质的识别辅助。 在这里我们描述了一种有效的方式来隔离粗糙的突触体,突触前分数和PSD分数。 它有助于确定突触蛋白的位置,并找到潜在的突触复杂

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