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Author:
Date:
2015-09-05
[Abstract] Here we describe the immunolocalization of a membrane-bound proton pump, the V-type H+-ATPase (VHA), in tissues and isolated cells of scleractinian corals. Immunolocalization of coral proteins requires additional steps not required for various model organisms, such as decalcification of the coral skeleton for immunohistochemistry or removal of cells away from the skeleton for immunocytochemistry. The tissue and cell preparation techniques described here can be adapted for localization of other coral proteins, provided the appropriate validation steps have been taken for the primary antibodies and species of coral used. These techniques are important for improving our understanding of coral cell physiology
[摘要] 在这里我们描述膜结合质子泵，V型H + -ATPase（VHA），在组织和分离的细胞的scleractinian珊瑚的免疫定位。 珊瑚蛋白的免疫定位需要对于各种模型生物不需要的额外步骤，例如用于免疫组织化学的珊瑚骨架的脱钙或从用于免疫细胞化学的骨架移除细胞。 本文所述的组织和细胞制备技术可适用于其它珊瑚蛋白的定位，条件是已对所用的一级抗体和珊瑚种类采取了适当的验证步骤。 这些技术对于提高我们对珊瑚细胞生理学的理解是重要的
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