| Immunolocalization of Proteins in Corals: the V-type H+-ATPase Proton Pump
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Author:
Date:
2015-09-05
[Abstract] Here we describe the immunolocalization of a membrane-bound proton pump, the V-type H+-ATPase (VHA), in tissues and isolated cells of scleractinian corals. Immunolocalization of coral proteins requires additional steps not required for various model organisms, such as decalcification of the coral skeleton for immunohistochemistry or removal of cells away from the skeleton for immunocytochemistry. The tissue and cell preparation techniques described here can be adapted for localization of other coral proteins, provided the appropriate validation steps have been taken for the primary antibodies and species of coral used. These techniques are important for improving our understanding of coral cell physiology
[摘要] 在这里我们描述膜结合质子泵，V型H + -ATPase（VHA），在组织和分离的细胞的scleractinian珊瑚的免疫定位。 珊瑚蛋白的免疫定位需要对于各种模型生物不需要的额外步骤，例如用于免疫组织化学的珊瑚骨架的脱钙或从用于免疫细胞化学的骨架移除细胞。 本文所述的组织和细胞制备技术可适用于其它珊瑚蛋白的定位，条件是已对所用的一级抗体和珊瑚种类采取了适当的验证步骤。 这些技术对于提高我们对珊瑚细胞生理学的理解是重要的
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| Spindle Angle Measurements
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Author:
Date:
2013-10-05
[Abstract] Spindle angles measures derive from the measures of spindle poles positions that were taken from fixed and immunostained adherent cells. To determine spindle angles (α), z-stack images of metaphasic cells immunostained with anti γ-tubulin (spindle poles) and anti β-tubulin antibodies (mitotic spindle) were acquired. A very simple ImageJ software macro was developed to measure the spindle angle using spindle pole coordinates (see Figure 1).
[摘要] 主轴角度测量值取自固定和免疫染色的贴壁细胞的纺锤体极点位置的测量值。 为了确定主轴角度（α），获得用抗γ-微管蛋白（纺锤极）和抗β-微管蛋白抗体（有丝分裂纺锤体）免疫染色的间期细胞的z-堆叠图像。 开发了一个非常简单的ImageJ软件宏，用主轴极坐标测量主轴角度（见图1）。
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