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Author:
Date:
2015-11-05
[Abstract] Mesenchymal stromal cells (MSCs) are non-hematopoietic, perivascular cells which support hematopoiesis and are thought to participate in tissue repair in vivo. MSCs can be isolated from various tissues and organs and are defined in vitro as plastic adherent cells expressing CD73, CD90, CD105 (human MSCs) or CD29, CD44, sca-1 (murine MSCs) which can differentiate into osteoblasts, adipocytes, chondroblasts and myocytes. MSCs possess potent immunomodulatory and trophic capacities in vitro and in vivo and have thus emerged as a promising treatment of inflammatory/autoimmune diseases. The use of MSCs for human disease relies on the injection of a large number of cells and much effort has been focused on acquiring MSCs with high proliferative capacity. Thus, ...
[摘要] 间充质基质细胞（MSC）是非造血的血管周围细胞，其支持造血并且被认为在体内参与组织修复。 MSC可以从各种组织和器官中分离并在体外定义为表达CD73，CD90，CD105（人MSC）或CD29，CD44，sca-1（鼠MSC）的塑性贴壁细胞，其可以分化成骨细胞，脂肪细胞，软骨细胞和肌细胞。 MSC在体外具有强的免疫调节和营养能力，并且在体内具有强的免疫调节能力，因此已经成为炎症/自身免疫疾病的有希望的治疗。 MSC对人类疾病的使用依赖于大量细胞的注射，并且许多努力已经集中在获得具有高增殖能力的MSC。因此，建立简单和准确的测定MSC增殖的协议对于基础研究和应用研究都是重要的。目前的方案提供了关于如何使用xCELLigence系统和CellTiter-Blue试剂（Carrillo-Galvez等人，）分离和测量源自腹股沟和/或腹内脂肪组织（mASCs）的鼠MSC的增殖的细节>，2015; Anderson ，，2013）。下面描述的方案也可以容易地翻译成人MSC。
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Author:
Date:
2015-08-05
[Abstract] This protocol aims to study intercellular transport of mitochondria, dynamic cellular organelles via tunnelling nanotubes (TNT), a cell membrane extension of cytoskeletal elements. The nanotubular bridges or the tunnelling nanotube highways are one of the emerging new cell-to-cell communication systems which mediates exchange of cellular materials, most importantly as in our observation, mitochondria. Mesenchymal stem cells (MSC) have been well studied to be endowed with a highly efficient intercellular mitochondrial donation ability and this property is now proven crucial to its functional role of rescue in cellular therapy.
[摘要] 此协议旨在研究线粒体，动态细胞器通过隧道纳米管（TNT），细胞骨架元素的细胞膜扩展的细胞间运输。 纳米管桥或隧道纳米管高速公路是新兴的新的细胞到细胞通信系统之一，其介导细胞材料的交换，最重要的是在我们的观察中，线粒体。 间充质干细胞（MSC）已经被充分研究以赋予高效的细胞间线粒体捐赠能力，并且这种性质现在被证明对于其在细胞治疗中的挽救的功能作用是至关重要的。
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