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Dextrose Glucose

葡萄糖葡萄糖

Company: BD
Catalog#: 215530
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Preparation of Candida albicans Biofilms for Transmission Electron Microscopy
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Date:
2013-07-20
[Abstract]  Transmission Electron Microscopy is a form of microscopy that allows for imaging of distinct portions of an individual cell. For Candida albicans biofilms, it is often used to visualize the cell walls of fixed samples of yeast and hyphae. This protocol describes how to grow, harvest, and fix Candida albicans biofilms in preparation for Transmission Electron Microscopy. [摘要]  透射电子显微术是允许对单个细胞的不同部分成像的显微镜检查的形式。 对于白色念珠菌生物膜,它通常用于可视化酵母和菌丝的固定样品的细胞壁。 此协议描述如何生长,收获和修复白色念珠菌生物膜准备透射电子显微镜。

Preparation of Candida albicans Biofilms Using an in vivo Rat Central Venous Catheter Model
Author:
Date:
2013-07-20
[Abstract]  In vivo biofilms grown on medical devices are necessary to understand the interactions of the fungal biofilm and the host environment in which it is most commonly found. This protocol describes a way to grow Candida albicans biofilms on the interior lumen of central venous catheters surgically implanted into rats, which mimics quite well the clinical cases of biofilms found on human central venous catheters. These infected catheters can then be studied via a multitude of different experiments, including cell counting by plating, imaging the catheters under light or electron microscopy, or comparing the relative content of in vivo biofilms to in vitro biofilms and planktonic cultures. These biofilms also provide enough high quality RNA for ... [摘要]  在医疗装置上生长的体内生物膜是必要的,以了解真菌生物膜和其中最常见的宿主环境的相互作用。 该协议描述了在手术植入大鼠中的中心静脉导管的内腔上生长白假丝酵母生物膜的方法,其相当好地模拟在人中心静脉导管上发现的生物膜的临床情况。 然后可以通过多种不同的实验研究这些感染的导管,包括通过电镀的细胞计数,在光学或电子显微镜下对导管成像,或者将体内生物膜的相对含量与 体外生物膜和浮游培养物。 这些生物膜还提供足够高质量的RNA进行转录分析。

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