Penicillin-Streptomycin (10,000 U/mL)
Company: Thermo Fisher Scientific
Catalog#: 15140122
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Protocol for Notch-ligand Binding Assays Using Dynabeads
Author:
Date:
2017-10-20
[Abstract]  This protocol describes how to measure interaction between Notch receptors and their ligands by cell-based assay using Dynabeads. We have used the protocol to determine binding capacity between Notch1-transfected HEK293T cells and ligand-coated Dynabeads. Expression of Eogt in Notch1-expressing cells promoted binding toward DLL4-coated beads, but not JAG1-coated beads. The Notch-ligand assay using Dynabeads suggested that Eogt facilitates DLL4-Notch1 interaction (Sawaguchi et ...

In vitro Co-culture of Mesenchymal Stem Cells and Endothelial Colony Forming Cells
Author:
Date:
2017-10-20
[Abstract]  The discovery of endothelial colony forming cells (ECFCs) with robust self-renewal and de novo vessel formation potentials suggests that ECFCs can be an excellent cell source for cardiovascular diseases treatment through improving neovascularization in the ischemic tissues. However, their engraftment after transplantation resulted to be low. Previous studies showed mesenchymal stem/stromal cells (MSCs) could improve the survival and capillary formation capacity of ECFCs in co-culture systems. ...

Isolation and Purification of Schwann Cells from Spinal Nerves of Neonatal Rat
Author:
Date:
2017-10-20
[Abstract]  Primary cultured Schwann cells (SCs) are widely used in the investigation of the biology of SC and are important seed cells for neural tissue engineering. Here, we describe a novel protocol for harvesting primary cultured SCs from neonatal Sprague-Dawley (SD) rats. In the present protocol, dissociated SCs are isolated from the spinal nerves of neonatal rats and purified by the treatment of cytosine arabinoside (AraC).

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