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Author:
Date:
2013-11-05
[Abstract] Chick embryos are known to be a powerful system to test gene function due to the “in vivo” accessibility, short time for results retrieval and the possibility to perform a large number of experiments. Synthetic micro-beads soaked in morphogenetic signals or receptor inhibitors can be implanted in selective embryo regions at precise developmental stages activating or blocking different signaling pathways. Here, we describe the manipulation of Wnt signaling pathway using Dkk-1-soaked micro-beads, implanted in ovo in the anterior part of the developing neural tube of chicken embryos; specifically at the prospective zona limitans intrathalamica at stage HH10.
[摘要] 已知小鸡胚胎是由于“体内”可及性，结果检索的时间短以及进行大量实验的可能性而测试基因功能的强大系统。 浸泡在形态发生信号或受体抑制剂中的合成微珠可以在激活或阻断不同信号通路的精确发育阶段植入选择性胚胎区域。 在这里，我们描述了使用Dkk-1浸泡的微珠的Wnt信号通路的操作，植入卵胚发育的神经管的前胚胎的胚胎; 特别是在HH10阶段的潜在可能范围内的脑脊液。
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