0.2 μM Polycarbonate 25 mm membranes

0.2μM聚碳酸酯25 mm膜

Company: Cytiva

Catalog#: K02BP02500

A Simple and Reproducible Stereomicroscopic Method to Assess Fungal Biofilms: Application to Antifungal Susceptibility Testing

Zinnat Shahina and Tanya E. S. Dahms

Published online: 2023-07-05

Bio-protocol()

Company-protocol()

Other protocol()

H₂O₂ Kill Assays of Biofilm Bacteria

Author:

Malika Khakimova and Dao Nguyen,

Date:

2013-11-05

[Abstract] Ubiquitous in nature and often surface associated, biofilms cause numerous chronic human infections. Biofilms are structured multicellular bacterial communities where cells are entrapped in a polymer matrix. Bacteria growing as biofilms are characterized by marked tolerance to many biocides, including oxidants such as hydrogen peroxide. Hydrogen peroxide is both produced by host phagocytic cells, and used as an antimicrobial compound. Understanding biofilm tolerance to hydrogen peroxide is therefore relevant to the persistence of Pseudomonas aeruginosa in human infections (such as chronic Pseudomonas aeruginosa infections in cystic fibrosis airways) as well as in environmental settings (such as water pipes).

This protocol was developed to determine the ... [摘要] 无处不在的自然和经常表面相关，生物膜导致许多慢性人类感染。生物膜是结构化的多细胞细菌群落，其中细胞被包埋在聚合物基质中。作为生物膜生长的细菌的特征在于对许多杀生物剂包括氧化剂如过氧化氢的显着耐受性。过氧化氢由宿主吞噬细胞产生，并用作抗微生物化合物。因此，了解对过氧化氢的生物膜耐受性与人类感染（例如囊性纤维化气道中的慢性绿脓假单胞菌感染）以及环境设置中的铜绿假单胞菌的持久性有关（如水管）。
该方案用于确定绿脓杆菌生物膜对过氧化氢（H 2 O 2 O 2）杀伤的耐受性。细菌作为菌落生物膜在聚碳酸酯膜上生长，如先前在Walters等人2003中所述。该方案可以适用于其它细菌，在H 2亚型中具有适当的变化， O 2浓度，因为不同的细菌物种可能比H 2 O 2比对绿脓杆菌更敏感或更不敏感。

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0.2 μM Polycarbonate 25 mm membranes

0.2μM聚碳酸酯25 mm膜

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