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Author:
Date:
2013-10-20
[Abstract] Gram-negative bacterial cells possess two membranes - the inner cytoplasmic membrane and the outer membrane. The two membranes are distinct in their composition; the inner membrane is composed of a phospholipid bilayer, whereas the outer membrane (OM) is composed of an asymmetrical bilayer, with the outer leaflet containing lipopolysaccharide (LPS) (Raetz and Whitfield, 2002). Surface polysaccharides, such as LPS O-antigen, or capsular polysaccharide, are often tightly associated with the OM (Whitfield, 2006). This tight association can be used to generate a rough quantification of surface polysaccharides of Gram-negative bacterial cells, as the OM can easily be dissociated from cells without associated cell lysis (Brimacombe et al., 2013). The following method describes how to ...
[摘要] 革兰氏阴性细菌细胞具有两个膜 - 内部细胞质膜和外部膜。 两种膜的组成是不同的; 内膜由磷脂双层组成，而外膜（OM）由不对称双层组成，外层含有脂多糖（LPS）（Raetz和Whitfield，2002）。 表面多糖，如LPS O-抗原或荚膜多糖，通常与OM紧密相关（Whitfield，2006）。 这种紧密关联可以用于产生革兰氏阴性细菌细胞的表面多糖的粗略定量，因为OM可以容易地从没有相关细胞裂解的细胞中解离（Brimacombe等人，2013）。 以下方法描述如何快速提取和定量OM相关多糖。
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