| Analysis of the Incorporation of Carbon Atoms from Radioactive Lactate into Proteins
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Author:
Date:
2013-07-05
[Abstract] This method allows to analyze if the carbon atoms of lactate are embedded into proteins. Indeed, mammalian cells express the transporter of monocarboxylic acids (called MCT1) that allows the entry of lactate into the cell. To this end, cells are incubated for 24 h with the culture medium containing lactate uniformly labeled with carbon 14 and then, lactate inside the cell is evaluated by counting the radioactivity by a scintillator.
[摘要] 该方法允许分析乳酸盐的碳原子是否嵌入蛋白质。 实际上，哺乳动物细胞表达允许乳酸进入细胞的单羧酸（称为MCT1）的转运蛋白。 为此，将细胞与含有用碳14均匀标记的乳酸盐的培养基一起温育24小时，然后通过用闪烁体计数放射性来评价细胞内的乳酸盐。
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| Detection of Released CO2 by Radioactive Lactate
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Author:
Date:
2013-07-05
[Abstract] This method allows to evaluate the degradation of lactate during cellular respiration. During this metabolic process, carbon atoms of lactate can be transformed in carbon dioxide. For this purpose, the radioactive lactate is added to the cells and the amount of radioactive carbon dioxide liberated is monitored. The radioactive carbon dioxide generated during cellular respiration is released into the culture medium and it is further converted into gas through the addition of sulfuric acid to culture media. A piece of Whatman paper wet with phenyl-ethylamine-methanol is placed inside the petri dish to trap radioactive carbon dioxide whose production is then evaluated by scintillator counting.
[摘要] 该方法允许在细胞呼吸期间评估乳酸盐的降解。 在这种代谢过程中，乳酸的碳原子可以转化为二氧化碳。 为此，将放射性乳酸盐加入到细胞中，并监测释放的放射性二氧化碳的量。 在细胞呼吸期间产生的放射性二氧化碳释放到培养基中，并通过向培养基中加入硫酸进一步转化为气体。 将一片用苯基 - 乙胺 - 甲醇湿润的Whatman纸置于培养皿内以捕集放射性二氧化碳，然后通过闪烁计数器评价其产量。
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