| Isolation and Primary Cell Culture of Mouse Dorsal Root Ganglion Neurons
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Author:
Date:
2016-04-05
[Abstract] We here provide a detailed protocol for the isolation and culture of primary mouse sensory neurons. The cell bodies of sensory afferent pseudounipolar neurons are located in dorsal root ganglia (DRGs) along the vertebral column. Dissected mouse DRGs can be dissociated into single cells by enzymatic digestion to obtain primary cultures of mouse sensory neurons as performed in the studies reported by Khaminets et al. (2015).
[摘要] 我们在这里提供了详细的协议，用于隔离和培养的主要小鼠感觉神经元。 感觉传入假性极化神经元的细胞体位于沿着脊柱的背根神经节（DRG）中。 解离的小鼠DRG可以通过酶消化解离成单个细胞，以获得小鼠感觉神经元的原代培养物，如Khaminets等人（2015）报道的研究中所进行的。
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| Immunoblot Analysis of Histone H4 Acetylation and Histone H2A Phosphorylation in Candida albicans
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Author:
Date:
2013-10-20
[Abstract] Posttranslational modifications of histones are required for different processes including transcription, replication and DNA damage repair. This protocol describes the preparation of a whole-cell extracts for the fungal pathogen Candida albicans. Furthermore, the extract is used to detect lysine acetylation of histone H4 as well as serine 129 phosphorylation of histone H2A by immunoblot analysis.
[摘要] 包括转录，复制和DNA损伤修复的不同过程需要组蛋白的翻译后修饰。 该方案描述了真菌病原体白色念珠菌的全细胞提取物的制备。 此外，提取物用于通过免疫印迹分析检测组蛋白H4的赖氨酸乙酰化以及组蛋白H2A的丝氨酸129磷酸化。
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| IFN-α Inhibition Assay in vitro
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Author:
Date:
2013-06-20
[Abstract] During viral infections Interferon-α (IFN-α) is expressed by infected host cells. IFN-α binds to its receptor (IFNAR1/2), which leads to the activation of downstream signaling via JAK-STAT. This signaling cascade results in the expression of several hundred different genes, so called interferon-stimulated gene, which lead to an antiviral state of the infected and the neighboring cells.
[摘要] 在病毒感染期间，干扰素-α（IFN-α）由感染的宿主细胞表达。 IFN-α结合其受体（IFNAR1/2），其导致通过JAK-STAT的下游信号传导的激活。 这种信号级联导致数百种不同基因的表达，所谓的干扰素刺激基因，其导致感染细胞和相邻细胞的抗病毒状态。
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