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Author:
Date:
2012-12-20
[Abstract] The production of hydrogen peroxide (H2O2) has been recognized as an important feature of plant cells that undergo programmed cell death (PCD) during host-pathogen interaction. Thordal-Christensen et al. (1997) first described a method using chemical 3,3-diaminobenzidine (DAB) to detect the presence and distribution of H2O2 in barley leaves challenged by the powdery mildew fungus (Thordal-Christensen et al., 1997). Since then, this method has been adapted to many other plant species for in situ detection of H2O2. Here, we describe a modified protocol to stain and visualize H2O2 production in wheat leaves during infection by the necrotrophic fungus, Stagonospora ...
[摘要] 过氧化氢（H 2 O 2 O 2）的产生已被认为是在宿主 - 病原体相互作用期间经历程序性细胞死亡（PCD）的植物细胞的重要特征。 Thordal-Christensen等人（1997）首先描述了使用化学3,3-二氨基联苯胺（DAB）检测H 2 O 2基团的存在和分布的方法， （Thordal-Christensen等人，1997）攻击的大麦叶中的抗性。 从那时起，该方法已经适应于许多其它植物物种用于原位检测H 2 O 2 O 2。 在这里，我们描述了在感染期间通过坏死性真菌，角叉状瘤或由产生的坏死营养效应物的浸润来染色和可视化小麦叶中H 2 O 2 O 2产生的修饰的方案 真菌。 该方法的简短版本已经在（Liu等人2012年）中报道。
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