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Dual-PAM-100 for measuring chlorophyll fluorescence

双PAM-100测量叶绿素荧光

Company: Heinz Walz
Catalog#: Dual-PAM-100
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Chlorophyll Fluorescence Measurements in Arabidopsis Wild-type and Photosystem II Mutant Leaves
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Date:
2015-07-20
[Abstract]  Chlorophyll fluorescence measurement is a widely used technique to determine photosynthetic performance. Light energy absorbed by a chlorophyll molecule can be dissipated by driving photochemical energy conversion, as heat in non-photochemical quenching processes, or it is re-emitted as fluorescence. The loss of light energy as chlorophyll fluorescence is primarily derived from photosystem II. Photosystem II is a thylakoid-embedded multiprotein complex which provides the high redox potential needed to oxidize water. Within photosystem II photons of light are captured and used to energize electrons. Energized electrons are fed into the linear electron transport chain and photosystem II replenishes lost electrons with electrons from splitting of water. Chlorophyll fluorescence yield can be ... [摘要]  叶绿素荧光测量是一种广泛使用的技术来确定光合性能。由叶绿素分子吸收的光能可以通过驱动光化学能量转化,作为非光化学淬灭过程中的热量而消散,或者其作为荧光再发射。作为叶绿素荧光的光能的损失主要来自光系统II。光系统II是类囊体嵌入的多蛋白复合物,其提供氧化水所需的高氧化还原电位。在光系统II中,光子被捕获并用于激发电子。激发的电子被馈送到线性电子传输链,并且光系统II用来自水分裂的电子补充丢失的电子。可以使用调制的荧光计设备定量叶绿素荧光产量。在这种装置中,调制的测量光束(以高频率打开和关闭)和仅由测量光激发的荧光的平行检测允许在光合(光化)光的存在下进行叶绿素荧光测量。此外,高强度但短持续时间的光闪烁(饱和脉冲)用于确定在黑暗和光适应叶中的最大荧光产量。在该方案中,给出接受拟南芥野生型叶的典型荧光图的程序。此外,该程序可用于基于各自的荧光图和数值来鉴定影响光系统II的拟南芥突变植物。

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