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MAS-coated glass slide (super frost)

MAS涂层玻璃载玻片(超级霜)

Company: Matsunami Glass
Catalog#: S9441
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Measurement of the Number of Peroxisomes
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2014-11-05
[Abstract]  This is the detailed protocol for the measurement of the number of peroxisomes described by Shibata et al. (2013). It is difficult to count the number of organelles in a cell because of the thickness of plant leaves. To overcome this challenge, protoplasts were isolated from leaves, and the number of peroxisomes per protoplast was counted. This method can be applied to other organelles such as mitochondria that are labeled with GFP or its derivatives. [摘要]  这是用于测量由Shibata等人(2013)描述的过氧化物酶体的数量的详细方案。 由于植物叶片的厚度,很难计数细胞中的细胞器的数目。 为了克服这种挑战,从叶中分离原生质体,并计数每个原生质体的过氧化物酶体的数目。 该方法可应用于用GFP或其衍生物标记的其他细胞器,例如线粒体。

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