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Author:
Date:
2013-12-20
[Abstract] Hepatitis C virus (HCV) is the main causative agent of chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma. Since the HCV genome is present exclusively in RNA form during replication, a number of anti-HCV drugs show appearance of rapid drug-resistant viruses. Therefore, it is important to test generation of drug-escape mutant viruses by developed antiviral drugs for their validity. Here, we describe a colony formation assay-based method to observe appearance of drug-resistant viruses against nucleic acid based anti-HCV drugs in genotype 1b based subgenomic replicon cell culture system (Lee et al., 2013).
[摘要] 丙型肝炎病毒（HCV）是慢性肝炎，肝硬化和肝细胞癌的主要致病因子。 由于HCV基因组在复制期间仅以RNA形式存在，许多抗HCV药物显示出快速耐药病毒的出现。 因此，重要的是通过开发的抗病毒药物来测试药物逃逸突变病毒的生成的有效性。 在这里，我们描述了基于集落形成测定的方法，观察耐药病毒对基于核酸的抗HCV药物在基于基因型1b的亚基因组复制子细胞培养系统中的外观（Lee等人，2013 ）。
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