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IgG from rabbit serum-10 mg

来自兔血清的IgG-10mg
Company: Sigma-Aldrich
Catalog#: I5006
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Generation and Maturation of Human Monocyte-derived DCs
Author:
Date:
2014-08-05
[Abstract]  Dendritic cells (DC) are antigen-presenting cells, which play a critical role in the regulation of the adaptive immune response. They act as a bridge between the innate and the adaptive immune systems. An approach to study their function and potentiality is to generate DC-like cells by culturing CD14+ monocyte-enriched peripheral blood mononuclear cells (PBMC). In the presence of GM-CSF and IL-4, these cultures give rise to large numbers of DC-like cells. Generating human-DC from PBMC is a useful tool to study biological functions of human DC. [摘要]  树突状细胞（DC）是抗原呈递细胞，其在适应性免疫应答的调节中起关键作用。 它们作为先天免疫系统和适应性免疫系统之间的桥梁。 研究它们的功能和潜力的方法是通过培养富集CD14 +单核细胞的外周血单核细胞（PBMC）产生DC样细胞。 在GM-CSF和IL-4存在下，这些培养物产生大量的DC样细胞。 从PBMC产生人类DC是研究人类DC的生物学功能的有用工具。

Rabbit IgG Conjugation to Dynabeads
Author:
Date:
2011-05-05
[Abstract]  This method couples rabbit IgG (or any other proteins serve as affinity reagent) to the surface of magnetic beads (Brand name: Dynabeads). The amine and thiol groups of amino acid residues on the protein are covalently linked with the epoxy group on Dynabeads. The coupled IgG beads can be stored at 4°C for 6-12 month with no obvious loss in reactivity. [摘要]  该方法将兔IgG（或任何其他蛋白质用作亲和试剂）偶联至磁珠（商品名：Dynabeads）的表面。 蛋白质上的氨基酸残基的胺基和硫醇基与Dynabeads上的环氧基共价连接。 偶联的IgG珠可以在4℃下储存6-12个月，没有明显的反应性损失。

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