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Sequencing Grade Modified Trypsin

测序级改性胰蛋白酶

Company: Promega
Catalog#: V5113
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In-Solution Digestion Of Purified Yeast Protein For LC-MS
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Date:
2011-04-20
[Abstract]  This method describes the preparation of total yeast protein extract for mass spectrometry analysis. The protein extract is digested by trypsin in a solution with strong denaturants. The digested sample is dried and re-constituted in a mixture compatible with HPLC separation. Samples of isobaric labels should be processed in parallel experiments starting from trypsin digestion. [摘要]  该方法描述了用于质谱分析的总酵母蛋白质提取物的制备。 蛋白质提取物通过胰蛋白酶在具有强变性剂的溶液中消化。 将消化的样品干燥并在与HPLC分离相容的混合物中重构。 同量异位标记样品应从胰蛋白酶消化开始在平行实验中进行处理。

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