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Thiazolyl Blue Tetrazolium Bromide

噻唑基蓝四唑溴化物

Company: Sigma-Aldrich
Catalog#: M5655
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MTT Assay of Cell Numbers after Drug/Toxin Treatment
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2011-04-05
[Abstract]  MTT assay is a colorimetric method for measuring the activity of enzymes in living cells that reduce MTT to formazan dyes, giving a purple color. It is commonly used to determine cytotoxicity of potential medicinal agents and toxic materials, since these types of materials are expected to stimulate or inhibit cell viability and growth. Here, a general protocol is described to carry out an MTT assay on different types of cells. [摘要]  MTT测定法是用于测量活细胞中酶的活性的比色法,其将MTT还原为甲an染料,得到紫色。 它通常用于确定潜在药剂和毒性材料的细胞毒性,因为这些类型的材料预期刺激或抑制细胞活力和生长。 这里,描述了对不同类型的细胞进行MTT测定的一般方案。

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