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Semi-micro HPLC system

半微量HPLC系统
Company: JASCO International
Catalog#: Micro21LC
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Detection of Hydroxyproline O-galactoside by LC/MS
Author:
Date:
2016-01-20
[Abstract]  Hydroxyproline (Hyp) O-galactosylation is a plant-specific post-translational modification found in extracellular glycoproteins such as arabinogalactan proteins (AGPs). Hyp O-galactosylation is mediated by Hyp O-galactosyltransferase (HPGT) that catalyzes the transfer of a D-galactopyranosyl residue to the hydroxyl group of Hyp residues of peptides from the sugar donor UDP-α-D-Gal. Here we describe an LC/MS-based method for the detection of Hyp O-galactoside. [摘要]  羟脯氨酸（Hyp）O - 半乳糖基化是在细胞外糖蛋白例如阿拉伯半乳聚糖蛋白（AGP）中发现的植物特异性翻译后修饰。 Hyp O - 半乳糖基化由Hyp O - 半乳糖基转移酶（HPGT）介导，其催化D-吡喃半乳糖残基转移到来自糖的肽的Hyp残基的羟基上 供体UDP-α-D-Gal。 在这里，我们描述了基于LC/MS的Hyp O /α-半乳糖苷的检测方法。

LC/MS-based Detection of Hydroxyproline O-galactosyltransferase Activity
Author:
Date:
2016-01-20
[Abstract]  Arabinogalactan proteins (AGPs) are plant-specific extracellular glycoproteins regulating a variety of processes during growth and development. AGP biosynthesis involves O-galactosylation of hydroxyproline (Hyp) residues followed by a stepwise elongation of the complex sugar chains. The initial Hyp O-galactosylation is mediated by Hyp O-galactosyltransferase (HPGT) that catalyzes the transfer of a D-galactopyranosyl residue to the hydroxyl group of Hyp residues of peptides from the sugar donor UDP-α-D-galactose (Figure 1). Here we describe a LC/MS-based method for the detection of HPGT activity in vitro.


Figure 1. Reaction scheme for Hyp ... [摘要]  阿拉伯半乳聚糖蛋白（AGP）是在生长和发育期间调节多种过程的植物特异性胞外糖蛋白。 AGP生物合成包括羟脯氨酸（Hyp）残基的O - 半乳糖基化，随后复合糖链的逐步延伸。 最初的Hyp O O - 半乳糖基化由Hyp O - 半乳糖基转移酶（HPGT）介导，其催化D-吡喃半乳糖残基转移到肽的Hyp残基的羟基上 糖供体UDP-α-D-半乳糖（图1）。 在这里，我们描述了用于在体外检测HPGT活性的基于LC/MS的方法。


图1. HPGT对Hyp半乳糖基化的反应方案。 HPGT催化从UDP-α-D-Gal向Hyp残基的羟基添加D-吡喃半乳糖。

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