Boric acid
Company: Sigma-Aldrich
Catalog#: B7660
Product evaluation:
Mutant Huntingtin Secretion in Neuro2A Cells and Rat Primary Cortical Neurons
Author:
Date:
2018-01-05
[Abstract]  Quantitative analysis of proteins secreted from the cells poses a challenge due to their low abundance and the interfering presence of a large amount of bovine serum albumin (BSA) in the cell culture media. We established assays for detection of mutant huntingtin (mHtt) secreted from Neuro2A cell line stably expressing mHtt and rat primary cortical neurons by Western blotting. Our protocol is based on reducing the amounts of BSA in the media while maintaining cell viability and secretory ...

Neuron Culture from Mouse Superior Cervical Ganglion
Author:
Date:
2014-01-20
[Abstract]  The rodent superior cervical ganglion (SCG) is a useful and readily accessible source of neurons for studying the mechanisms of sympathetic nervous system (SNS) development and growth in vitro. The sympathetic nervous system (SNS) of early postnatal animals undergoes a great deal of remodeling and development; thus, neurons taken from mice at this age are primed to re-grow and establish synaptic connections after in situ removal. The stereotypic location and size of the SCG ...

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