Sonicator (Probe size, 3 mm)

超声波仪（探头尺寸，3 mm）

Company: Sonics & Materials

Catalog#: VLB 3X

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Analysis of Lipid-linked Oligosaccharides Synthesized in vivo in Schizosaccharomyces pombe

Ayelen Valko, Giovanna L. Gallo, Ariel D. Weisz, Armando J. Parodi and Cecilia D’Alessio

Published online: 2022-09-20

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Determination of Keto-deoxy-d-manno-8-octanoic acid (KDO) from Lipopolysaccharide of Escherichia coli

Author:

M. R. Sunayana and Manjula Reddy,

Date:

2015-12-20

[Abstract] 2-Keto-3-deoxy-octonate (KDO) is an essential constituent of lipopolysaccharide (LPS) that forms the outermost leaflet of Gram-negative bacterial outer membrane. LPS is mainly composed of lipid A, O-antigen and a core oligosaccharide. Two molecules of KDO are present per one molecule of LPS. A proper level of LPS is required to maintain the outer membrane integrity and either high or low levels of LPS are toxic to the cell. Various methods are available for quantification of LPS; of these, determination of KDO is a simple and accurate method and it can be estimated either directly from crude bacterial cell lysates or from purified LPS by a simple colorimetric assay. Although this procedure can be theoretically used for any Gram-negative bacterium, we used it routinely to measure KDO from ... [摘要] 2-酮-3-脱氧 - 辛酸（KDO）是形成革兰氏阴性细菌外膜的最外侧叶的脂多糖（LPS）的必需成分。 LPS主要由脂质A，O-抗原和核心寡糖组成。每一分子LPS存在两个KDO分子。需要适当水平的LPS以维持外膜完整性，并且高或低水平的LPS对细胞有毒性。各种方法可用于定量LPS;其中，KDO的测定是简单和准确的方法，并且可以通过简单的比色测定直接从粗制细菌细胞裂解物或从纯化的LPS估计。尽管该方法可以理论上用于任何革兰氏阴性菌，我们常规地使用它来测量来自大肠杆菌（大肠杆菌）K12菌株的细胞裂解物的KDO。 br /> 方法：协议取自Karkhanis等人（1978）。它是一种测量KDO的简单，灵敏和可靠的方法。该测定在细胞裂解物或LPS完全酸水解后释放LPS的各种组分进行。此外，与高碘酸盐，亚砷酸盐和硫代巴比妥酸的反应产生粉红色至红色的发色团，其在用DMSO稳定后在548nm测量。

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Sonicator (Probe size, 3 mm)

超声波仪（探头尺寸，3 mm）

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