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Mouse anesthesia system with isoflurane box

研究ANESTHESIA机器

Company: Parkland Scientific
Catalog#: V3000PK
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H1N1 Virus Production and Infection
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Date:
2018-10-20
[Abstract]  Influenza A virus is a member of orthomyxoviridae family causing wide-spread infections in human respiratory tract. Mouse infection model is widely used in antiviral research and pathogenesis study against influenza A virus. Here, we report a protocol in infected mice with different virus doses and strains to explore how an inhibitor of lysine-specific demethylase (LSD1) impacts disease progression. [摘要]  甲型流感病毒是正粘病毒科的成员,在人呼吸道中引起广泛的感染。 小鼠感染模型广泛用于抗甲型流感病毒的抗病毒研究和发病机制研究。 在这里,我们报告了受感染小鼠中具有不同病毒剂量和菌株的方案,以探索赖氨酸特异性去甲基化酶(LSD1)的抑制剂如何影响疾病进展。

【背景】甲型流感病毒是正粘病毒科的一员,是一种负义RNA病毒,具有八个分段的单链病毒RNA(vRNA)基因组,编码10种以上的蛋白质。在过去100年中,流感病毒株的爆发经常出现在人群中,包括1918年由H1N1亚型引起的“西班牙流感”,1957年由H2N2引起的“亚洲流感”,1968年由H3N2引起的“香港流感”, “H1N1流感”于1977年发生的“俄罗斯流感”,以及2009年H1N1流行的“猪流感”(Smith et al。>,2009; Lim and Mahmood,2011; Kumar et al。> ,2018年)。季节性甲型流感病毒也在世界范围内传播,容易在人与人之间传播,每年导致全世界300万至500万人住院(Molinari et al。>,2007)。季节性流感感染每年导致290,000-650,000人死亡,主要是年幼儿童,老年人和重症患者(Kumar et al。>,2018)。

流感病毒研究中使用动物模型不仅可以阐明影响疾病结果的病毒和宿主因素,还可以在易感宿主中传播,还可以评估旨在预防或降低流感发病率和死亡率的干预措施(Thangavel和Bouvier,2014)。在本文中,我们使用两种甲型流感病毒,A ...

Analysis of Intestinal Permeability in Mice
Author:
Date:
2014-11-20
[Abstract]  The intestinal epithelial layer serves as a barrier against pathogens and ingested toxins, which are present in the lumen of the intestine. The importance of the intestinal epithelial barrier is emphasized by the alterations in paracellular permeability and tight junction functions observed in inflammatory bowel disease (IBD) and colon cancer. [摘要]  肠上皮层用作针对存在于肠腔中的病原体和摄入的毒素的屏障。 肠上皮屏障的重要性通过在炎症性肠病(IBD)和结肠癌中观察到的细胞旁通透性和紧密连接功能的改变来强调。

Evaluation of Caspase-1 Activation and IL-1β Production in A Kainic Acid Microdyalisis Brain Injury Model
Author:
Date:
2013-04-20
[Abstract]  Intracerebral infusion of kainic acid (KA) by a microdialysis probe induces a focal swelling in the brain-perfused area which promotes inflammation (Compan et al., 2012; Oprica et al., 2003). The microdialysis technique allows the local in vivo perfusion of KA and the simultaneous collection of inflammatory mediators, and other neuroactive substances, released in the injured brain. This protocol also allows the perfusion of different solutions in each cerebral hemisphere at the same time. By perfusing KA in isotonic solution of Krebs-Ringer Bicarbonate (KRB) (280-290 mOsm) in one hippocampus and KA in hypertonic KRB solution (1,400-1,500 mOsm) in the contralateral side, we can evaluate in vivo the efficiency of hypertonic solutions in preventing ... [摘要]  通过微透析探针脑内输注红藻氨酸(KA)在脑灌注区域诱导局灶性肿胀,这促进炎症(Compan等人,2012; Oprica等人, 。,2003)。微透析技术允许局部的体内灌注KA和同时收集炎性介质和其他神经活性物质,释放在受伤的脑中。该协议还允许在每个大脑半球同时灌注不同的溶液。通过在对侧中在一个海马中的Krebs-Ringer碳酸氢盐(KRB)(280-290mOsm)的等渗溶液中灌注KA和在高渗KRB溶液(1,400-1,500mOsm)中的KA,我们可以在体内评价高渗溶液在KA输注后预防由肿胀引起的炎症的效率。一旦已经诱导炎症反应,就有可能通过微透析探针输入胱天蛋白酶-1的生物素化特异性抑制剂,从而允许检测响应于损伤的IL-1产生的脑区域和细胞(Oprica等人, et al。,2003)。

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