{{'Search' | translate}}
 

Silicon grease

硅脂

Company: Dow Corning
Catalog#: Z273554
Bio-protocol()
Company-protocol()
Other protocol()

In vivo Imaging of Tumor and Immune Cell Interactions in the Lung
Author:
Date:
2016-10-20
[Abstract]  Immunotherapy has demonstrated great therapeutic potential by activating the immune system to fight cancer. However, little is known about the specific dynamics of interactions that occur between tumor and immune cells. In this protocol we describe a novel method to visualize the interaction of tumor and immune cells in the lung of live mice, which can be applied to other organs. In this protocol fluorescent-labeled tumor cells are transferred to recipient mice expressing fluorescently tagged immune cells. Tumor-immune cell interactions in the lung are then imaged by confocal or two photon microscopy. Analysis of tumor interactions with immune cells using this protocol should aid in a better understanding of the importance of these interactions and their role in developing ... [摘要]  免疫治疗已经通过激活免疫系统抵抗癌症而显示出巨大的治疗潜力。然而,关于在肿瘤和免疫细胞之间发生的相互作用的具体动力学知之甚少。在这个协议中,我们描述了一种新的方法来可视化肿瘤和免疫细胞在活小鼠的肺中的相互作用,可以应用到其他器官。在该协议中,荧光标记的肿瘤细胞转移到表达荧光标记的免疫细胞的受体小鼠。然后通过共聚焦或双光子显微镜对肺中的肿瘤 - 免疫细胞相互作用成像。使用该方案分析肿瘤与免疫细胞的相互作用应当有助于更好地理解这些相互作用的重要性及其在开发免疫疗法中的作用。

[背景] ...

Quantifying the Permeability of the Apoplastic Water Barrier in Cosmos Petals
Author:
Date:
2015-11-20
[Abstract]  The capacity of plants to minimize uncontrolled water loss is essential for survival in adverse and changing climatic conditions. In order to assess and compare the effectiveness of apoplastic barriers to water, the permeability of the barrier must first be quantified. Studies have accomplished this directly by quantifying tritium flux or indirectly by measuring the influx/efflux of water surrogates such as dyes, chlorophyll, and herbicides. Other studies have relied on comparative methods such as survival rates after drought. These methods rely on radioactive material, correlations, or qualitative comparisons. However, a quantitative method is necessary that directly measures water efflux and that allows easy comparisons within and between experiments, plant parts, plant species, and ... [摘要]  植物使不受控制的水损失最小化的能力对于在不利和变化的气候条件下的存活是必要的。为了评估和比较非水性阻隔层对水的有效性,必须首先量化阻隔层的渗透性。研究直接通过量化氚通量或通过测量水替代物如染料,叶绿素和除草剂的流入/流出间接实现了这一点。其他研究依赖于比较方法,如干旱后的存活率。这些方法依赖于放射性材料,相关性或定性比较。然而,定量方法是必要的,直接测量水流出,并允许在实验,植物部分,植物物种,特别是研究实验室内和之间的简单比较。这里我们详细介绍了Schönherr和Lendzian(1981)首次描述的重量分析方法,它可以在不到半天内完成,并且根据植物屏障在一到十天内完成。这种方法已经用于许多关于叶和果皮表皮的研究中,并且最近也用于来自宇宙的花瓣( Cosmos bipinnatus ; Buschhaus ,2015)。

Comments