|
Author:
Date:
2015-10-20
[Abstract] A plethora of natural products, mostly secondary metabolites, are isolated and purified from many different organisms, like plants, fungi, algae, marine invertebrates, etc. The extraction procedure is specific to each organism, but some guidelines are usually followed for any purification procedure regarding targeted metabolites, such as alkaloids. Alkaloids are secondary metabolites that contain basic nitrogen in their structures and they are often associated with interesting biological properties especially in pharmacology field. This protocol describes the isolation procedure of indole alkaloids from Rauvolfia nukuhivensis directly from the ethanol extract of the plant material yielding different skeleton-type compounds including non-basic derivatives (ajmaline, ...
[摘要] 从许多不同的生物体如植物，真菌，藻类，海洋无脊椎动物等分离和纯化了大量天然产物，主要是次级代谢物。提取程序对每个生物体是特异性的，但是关于靶向代谢物（例如生物碱）的任何纯化程序通常遵循一些指导。生物碱是在其结构中含有碱性氮的次级代谢物，并且它们通常与有趣的生物学性质相关，特别是在药理学领域。该方案描述了直接从植物材料的乙醇提取物中的来自真鞭毛虫的吲哚生物碱的分离过程，产生不同的骨架类化合物，包括非碱性衍生物（水杨酸，胭脂碱，大线和β-咔啉）。该程序详述了使用光谱技术（NMR，MS，UV）从植物收集到分子水平表征新的或已知的分离的化合物的指南和步骤。我们详细描述了化合物纯化之后的萃取和分馏方法，以及它们的物理化学表征。该程序通过从大白菜Rauvolfia nukuhivensis的树皮中纯化大量吲哚生物碱的实例来说明。
|