| Visualization of Cell Complexity in the Filamentous Cyanobacterium Mastigocladus laminosus by Transmission Electron Microscopy (TEM)
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Author:
Date:
2014-12-05
[Abstract] The cyanobacterium Mastigocladus laminosus (M. laminosus) is one of the most morphologically complex prokaryotes. It forms long chains of cells that are connected via septal junction complexes; such complexes allow diffusion of metabolites and regulators between neighboring cells. Cellular division occurs in multiple planes, resulting in the formation of true branches, and cell differentiation leads to the formation of specialized cell types for nitrogen fixation (heterocysts) and culture dispersal (hormogonia and necridia). Here, we describe a detailed protocol for the preparation of M. laminosus for TEM in order to visualize the ultrastructural properties of the organism. The presented preparation method is based on adding potassium permanganate as fixative ...
[摘要] 蓝藻（Mastigocladus laminosus）（ M。laminosus ）是最具形态复杂的原核生物之一。 它形成通过间隔连接复合物连接的细胞的长链; 这种复合物允许代谢物和调节剂在相邻细胞之间扩散。 细胞分裂发生在多个平面，导致真正的分支的形成，细胞分化导致固氮（heterocysts）和文化扩散（hormogonia和necridia）的专门细胞类型的形成。 在这里，我们描述了用于制备 M的详细方案。 层状结构用于TEM，以便可视化生物体的超微结构特性。 所提出的制备方法基于添加高锰酸钾作为固定剂，已显示增加膜的对比度（Luft，1956），使其适合于在其中光合膜的可视化是重要的蓝细菌的研究。
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| Immunoelectron Microscopy in Chlamydomonas Cells
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Author:
Date:
2013-03-05
[Abstract] The method of immunoelectron microscopy is intended for localization of proteins inside the cells of Chlamydomonas reinhardtii or other microalgae and cyanobacteria. This protocol was used to study localization of carbonic anhydrase Cah3 with antibodies raised in rabbit, though it can be used to localize any other abundant protein. Primary rabbit antibodies are recommended because they react quickly and specifically with proteins of C. reinhardtii. If primary antibodies other than rabbit are used, the blocking procedure and time of incubation with primary and secondary antibodies should be adjusted.
[摘要] 免疫电子显微镜法的目的是将蛋白质定位在莱茵衣藻或其它微藻和蓝细菌的细胞内。 该方案用于研究碳酸酐酶Cah3与在兔中产生的抗体的定位，尽管其可以用于定位任何其他丰富的蛋白质。 建议使用初级兔抗体，因为它们能快速和特异性地与C的蛋白反应。 reinhardtii 。 如果使用除兔以外的第一抗体，应当调整与第一和第二抗体孵育的封闭程序和时间。
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