| Measurement of Arabidopsis thaliana Plant Traits Using the PHENOPSIS Phenotyping Platform
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Author:
Date:
2018-02-20
[Abstract] High-throughput phenotyping of plant traits is a powerful tool to further our understanding of plant growth and its underlying physiological, molecular, and genetic determinisms. This protocol describes the methodology of a standard phenotyping experiment in PHENOPSIS automated platform, which was engineered in INRA-LEPSE (https://www6.montpellier.inra.fr/lepse) and custom-made by Optimalog company. The seminal method was published by Granier et al. (2006). The platform is used to explore and test various ecophysiological hypotheses (Tisné et al., 2010; Baerenfaller et al., 2012; Vile et al., 2012; Bac-Molenaar et al., 2015; Rymaszewski et al., 2017). Here, the focus ...
[摘要] 植物性状的高通量表型分析是进一步了解植物生长及其潜在生理，分子和遗传决定论的有力工具。该协议描述了在INRA-LEPSE（ https： //www6.montpellier.inra.fr/lepse ）并由Optimalog公司定制。开创性的方法由Granier等人发表（2006年）。该平台用于探索和测试各种生理生理假设（Tisnéet al。，2010; Baerenfaller et al。，2012; Vile et al。 > 2012; Bac-Molenaar 等，2015; Rymaszewski 等，2017）。在这里，重点关注实验的准备和管理，以及与生长相关的性状（例如，预测莲座面积，总叶面积和生长速率），水状况相关性状（<例如叶干物质含量和相对含水量）以及与植物结构相关的性状（例如气孔密度和指数以及叶片/叶柄比率）。简而言之，在生长室中设置完全随机的（块）设计。接下来，准备基底，测量其初始含水量并填充盆。种子播种到土壤表面并在实验之前发芽。发芽后，用户计划土壤浇水和图像（可见光，红外线，荧光）采集，并由自动机执行。在实验过程中可能会进行破坏性测量。从图像中提取数据并估计与生长相关的性状值涉及半自动程序和统计处理。 ...
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| Quantitative Evaluation of Competitive Nodulation among Different Sinorhizobium Strains
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Author:
Date:
2015-08-05
[Abstract] Legumes play a vital role in global food supply because they are uniquely capable of fixing atmospheric nitrogen (N) through symbioses with root and stem nodule bacteria, collectively called the rhizobia. These commonly include bacteria in the genera Rhizobium, Mesorhizobium, Sinorhizobium (Ensifer), and Bradyrhizobium, although other genera of bacteria have now been shown to form root nodule symbioses with several legume species (Weir, 2012). The symbiotic interaction is important for agricultural productivity, especially in less developed countries where nitrogen fertilizer is expensive. However, nodulation ability and competitiveness have practical importance in agricultural production, because the inoculation of efficient rhizobia is often ...
[摘要] 豆类在全球食物供应中起着至关重要的作用，因为它们独特地能够通过与根和茎结节细菌的共生固定大气氮（N），统称为根瘤菌。这些通常包括根瘤菌属，中生根瘤菌属，中华根瘤菌属（ Ensifer ）和 Bradyrhizobium 属中的细菌，的细菌现已显示与几种豆科物种形成根瘤共生（Weir，2012）。共生相互作用对于农业生产力是重要的，特别是在其中氮肥昂贵的欠发达国家中。然而，结瘤能力和竞争力在农业生产中具有实际重要性，因为高效根瘤菌的接种通常不成功，这主要是由于土壤中存在无效的土着根瘤菌的竞争性群体（Toro，1996; Triplett和Sadowsky， 。该协议允许我们定量评价中华根瘤菌菌株的相对结瘤竞争性。
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