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Company: Thermo Fisher Scientific
Catalog#: 19959
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Primer Extension Analysis of HBV DNA with Strand-Specific Primers
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2015-08-05
[Abstract]  We performed primer extension assay to determine which steps of HBV DNA synthesis (i.e., minus- and plus-strand DNA synthesis and circularization of RC DNA) are affected by phosphoacceptor site mutations in C protein. In these experiments, we used several specific oligonucleotide primers. For quantitation, the level of extended DNA (ED) was normalized to the level of a single internal standard (IS) DNA. [摘要]  我们进行引物延伸测定以确定HBV DNA合成的哪些步骤(即,负链和正链DNA合成和RC DNA的环化)受C蛋白中的磷酸受体位点突变的影响。 在这些实验中,我们使用几种特异性寡核苷酸引物。 为了定量,将延伸DNA(ED)的水平标准化为单个内标(IS)DNA的水平。

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