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Collagenase, Type 3 1 gm L

胶原酶,类型3 1 gm L

Company: Worthington Biochemical
Catalog#: LS004182
Other protocol()

Determining Leukocyte Origins Using Parabiosis in the PyMT Breast Tumor Model
[Abstract]  Tumors develop in a complex microenvironment alongside numerous cell types that impact their survival. Immune cells make up a large proportion of these accessory cells and many are known to promote tumor progression. Macrophages, in particular, are associated with poor patient prognosis and are therefore potential candidates for therapeutic targeting in cancer. However, to develop successful strategies to target macrophages, it is important to clarify whether these cells are derived from blood-borne precursors or a tissue-resident population. Parabiosis, or the surgical connection of two mice resulting in a shared blood circulation, allows the distinction between these two cellular sources. Here, we describe the use of parabiosis to define cell ontogeny in a mouse model of breast cancer. [摘要]  肿瘤在复杂的微环境中发展,伴随许多影响其存活的细胞类型。 免疫细胞构成这些附属细胞的大部分,并且已知许多细胞促进肿瘤进展。 巨噬细胞,特别是与不良的患者预后相关,因此是癌症中治疗靶向的潜在候选者。 然而,要开发成功的策略,以靶向巨噬细胞,重要的是要澄清这些细胞是否源自血源性前体或组织驻留人群。 Parabiosis或两个小鼠的外科连接导致共享的血液循环,允许这两个细胞来源之间的区别。 在这里,我们描述在乳腺癌的小鼠模型中定义细胞个体发育的拟杆菌的使用。

Neuron Culture from Mouse Superior Cervical Ganglion
[Abstract]  The rodent superior cervical ganglion (SCG) is a useful and readily accessible source of neurons for studying the mechanisms of sympathetic nervous system (SNS) development and growth in vitro. The sympathetic nervous system (SNS) of early postnatal animals undergoes a great deal of remodeling and development; thus, neurons taken from mice at this age are primed to re-grow and establish synaptic connections after in situ removal. The stereotypic location and size of the SCG make it ideal for rapid isolation and dissociation. The protocol described here details the requirements for the dissection, culture and differentiation of SCG neurons. The protocol is suitable for culturing neurons from late embryonic gestation to approximately postnatal day 3. The culture technique ... [摘要]  啮齿动物上级子宫颈神经节(SCG)是一种有用且易于获取的神经元来源,用于研究交感神经系统(SNS)体外发育和生长的机制。 早期出生后动物的交感神经系统(SNS)经历了大量的重塑和发育; 因此,在这个年龄的小鼠中取出的神经元被引发再生长并在原位移除后建立突触连接。 SCG的刻板位置和尺寸使其成为快速隔离和解离的理想选择。 这里描述的方案详述了SCG神经元的解剖,培养和分化的要求。 该方案适用于从晚期胚胎妊娠至大约出生后第3天培养神经元。下面讨论的培养技术利用玻璃盖玻片进行固定细胞的显微镜检查。