Measurement of Airway Responsiveness in the Anesthetized Mouse
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Author:
Date:
2013-04-05
[Abstract] Airway hyperresponsiveness to methacholine is an important characteristic of asthma. Many devices can be used to measure airway responsiveness in the mouse but it is well established that the invasiveness of a measurement technique is correlated with its precision and reproducibility. This protocol describes how to measure airway responses to aerosolized methacholine in anesthetized, tracheotomized, and mechanically ventilated mice using the forced oscillation technique with FlexiVent® (SCIREQ). This is a computer-controlled precision piston pump that can intersperse mechanical ventilation with volume and pressure controlled manoeuvres to obtain accurate, reproducible measurement of respiratory mechanics.
[摘要] 气道对乙酰甲胆碱的高反应性是哮喘的一个重要特征。 许多装置可以用于测量小鼠中的气道反应性,但是已经确定的是,测量技术的侵入性与其精确度和再现性相关。 该协议描述了如何使用强制振荡技术用FlexiVent </sup>(SCIREQ)测量在麻醉,气管切开和机械通气的小鼠中气雾化的乙酰甲胆碱的气道反应。 这是一个计算机控制的精密活塞泵,可以穿插机械通气与体积和压力控制操作,以获得准确,可重复的呼吸力学测量。
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Measurement of Airway Responsiveness on Vigil and Unrestrained Mouse
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Author:
Date:
2013-02-20
[Abstract] Airway hyperresponsiveness to methacholine is an important characteristic of asthma. This protocol describes how to measure airway response to methacholine in vigil and unrestraint mouse. The enhanced pause (PenH) is an index of the airway response to increasing doses of methacholine recorded by whole-body barometric plethysmography.
[摘要] 气道对乙酰甲胆碱的高反应性是哮喘的一个重要特征。 该协议描述如何测量在守夜和无约束小鼠中对乙酰甲胆碱的气道反应。 增强的暂停(PenH)是通过全身气压体积描记法记录的乙酰甲胆碱增加剂量的气道反应的指数。
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